Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris
De, Sonakshi (BOKU University of Natural Resources and Life Sciences Vienna. Department of Biotechnology)
Mattanovich, Diethard (BOKU University of Natural Resources and Life Sciences Vienna. Department of Biotechnology)
Ferrer, Pau (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Gasser, Brigitte (BOKU University of Natural Resources and Life Sciences Vienna. Christian Doppler Laboratory for growth-decoupled protein production in yeast)

Date:	2021
Abstract:	Besides bakers' yeast, the methylotrophic yeast Komagataella phaffii (also known as Pichia pastoris) has been developed into the most popular yeast cell factory for the production of heterologous proteins. Strong promoters, stable genetic constructs and a growing collection of freely available strains, tools and protocols have boosted this development equally as thorough genetic and cell biological characterization. This review provides an overview of state-of-the-art tools and techniques for working with P. pastoris, as well as guidelines for the production of recombinant proteins with a focus on small-scale production for biochemical studies and protein characterization. The growing applications of P. pastoris for in vivo biotransformation and metabolic pathway engineering for the production of bulk and specialty chemicals are highlighted as well.
Grants:	Agencia Estatal de Investigación PID2019-104666GB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1462
Rights:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Metabolic engineering ; Recombinant proteins ; Synthetic biology ; Systems biology ; Yeast
Published in:	Essays in Biochemistry, Vol. 65, Issue 2 (July 2021) , p. 293-307, ISSN 1744-1358

DOI: 10.1042/EBC20200138
PMID: 33956085

Postprint 30 p, 2.4 MB

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