Impact of Ag2S NPs on soil bacterial community : a terrestrial mesocosm approach
Lopes Peixoto, Sara Cristiana (Universidade de Aveiro. Centro de Estudos do Ambiente e do Mar)
Khodaparast, Zahra (Universidade de Aveiro. Centro de Estudos do Ambiente e do Mar)
Cornelis, Geert (Swedish University of Agricultural Sciences. Department of Soil and Environment)
Lahive, Elma (UK Centre for Ecology and Hydrology, Maclean Building)
Green Etxabe, Amaia (UK Centre for Ecology and Hydrology, Maclean Building)
Baccaro, Marta (Wageningen University. Division of Toxicology)
Papadiamantis, Anastasios G. (NovaMechanics Ltd)
Gonçalves, Sandra F.. (Universidade de Aveiro. Centro de Estudos do Ambiente e do Mar)
Lynch, Iseult (UK Centre for Ecology and Hydrology, Maclean Building)
Busquets-Fité, Martí (Applied Nanoparticles SL.)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Loureiro, Susana (Universidade de Aveiro. Centro de Estudos do Ambiente e do Mar)
Henriques, I. (Universidade de Coimbra. Departamento de Ciências da Terra)

Data:	2020
Resum:	Soils might be a final sink for AgS nanoparticles (NPs). Still, there are limited data on their effects on soil bacterial communities (SBC). To bridge this gap, we investigated the effects of AgS NPs (10 mg kg soil) on the structure and function of SBC in a terrestrial indoor mesocosm, using a multi-species design. During 28 days of exposure, the SBC function-related parameters were analysed in terms of enzymatic activity, community level physiological profile, culture of functional bacterial groups [phosphorous-solubilizing bacteria (P-SB) and heterotrophic bacteria (HB)], and SBC structure was analysed by 16S rRNA gene-targeted denaturing gradient gel electrophoresis. The SBC exposed to AgS NPs showed a significative decrease of functional parameters, such as β-glucosidase activity and L-arginine consumption, and increase of the acid phosphatase activity. At the structural level, significantly lower richness and diversity were detected, but at later exposure times compared to the AgNO treatment, likely because of a low dissolution rate of AgS NPs. In fact, stronger effects were observed in soils spiked with AgNO, in both functional and structural parameters. Changes in SBC structure seem to negatively correlate with parameters related to phosphorous (acid phosphatase activity) and carbon cycling (abundance of HB, P-SB, and β-glucosidase activity). Our results indicate a significant effect of AgS NPs on SBC, specifically on parameters related to carbon and phosphorous cycling, at doses as low as 10 mg kg soil. These effects were only observed after 28 days, highlighting the importance of long-term exposure experiments for slowly dissolving NPs.
Ajuts:	European Commission 646002
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Silver sulfide nanoparticle ; Oil bacterial community ; Denaturing gradient gel electrophoresis ; Soil enzymatic activity ; Community level physiological profile ; Indoor Mesocosm
Publicat a:	Ecotoxicology and Environmental Safety, Vol. 206 (Dec. 2020) , art. 111405, ISSN 1090-2414

DOI: 10.1016/j.ecoenv.2020.111405

12 p, 2.6 MB

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