Repair of Long Peripheral Nerve Defects in Sheep : A Translational Model for Nerve Regeneration
Contreras, Estefania (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Traserra Call, Sara (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Bolívar Martín, Sara (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
Forés, Joaquím (Hospital Clínic i Provincial de Barcelona)
José Cunilleras, Eduard (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Delgado Martínez, Ignacio (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
García Arnas, Félix (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Udina i Bonet, Esther (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)
Navarro, X. (Xavier) (Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas)

Date:	2023
Abstract:	Despite advances in microsurgery, full functional recovery of severe peripheral nerve injuries is not commonly attained. The sheep appears as a good preclinical model since it presents nerves with similar characteristics to humans. In this study, we induced 5 or 7 cm resection in the peroneal nerve and repaired with an autograft. Functional evaluation was performed monthly. Electromyographic and ultrasound tests were performed at 6. 5 and 9 months postoperation (mpo). No significant differences were found between groups with respect to functional tests, although slow improvements were seen from 5 mpo. Electrophysiological tests showed compound muscle action potentials (CMAP) of small amplitude at 6. 5 mpo that increased at 9 mpo, although they were significantly lower than the contralateral side. Ultrasound tests showed significantly reduced size of tibialis anterior (TA) muscle at 6. 5 mpo and partially recovered size at 9 mpo. Histological evaluation of the grafts showed good axonal regeneration in all except one sheep from autograft 7 cm (AG7) group, while distal to the graft there was a higher number of axons than in control nerves. The results indicate that sheep nerve repair is a useful model for investigating long-gap peripheral nerve injuries.
Grants:	European Commission. Horizon 2020 899822 Ministerio de Ciencia, Innovación y Universidades SAF2017-84464R Instituto de Salud Carlos III RD21/0017/008
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Large animal model ; Long gap ; Nerve injury ; Nerve regeneration ; Sheep
Published in:	International journal of molecular sciences, Vol. 24 (january 2023) , ISSN 1422-0067

DOI: 10.3390/ijms24021333
PMID: 36674848

17 p, 11.0 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Articles > Research articles
Articles > Published articles