Changes in biomarkers of redox status in serum and saliva of dogs with hypothyroidism
Arostegui, Luis G. González (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Muñoz Prieto, Alberto (University of Zagreb. Clinic for Internal Diseases)
Marín, Luis Pardo (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
López, Gregorio García (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Tvarijonaviciute, Asta (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Cerón, José Joaquín (Universidad de Murcia. Laboratorio Interdisciplinario de Análisis Clínicos Interlab)
Rubio, Camila Peres (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Data:	2023
Resum:	Hypothyroidism is the most common endocrine disorder diagnosed in dogs, leading to deleterious effects on a dog's life quality. This study aims to evaluate changes in the redox status in canine hypothyroidism. For this purpose, a comprehensive panel of antioxidants and oxidants biomarkers were measured in serum and saliva of 23 dogs with hypothyroidism, 21 dogs with non-thyroidal illness, and 16 healthy dogs. Among the antioxidants, cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol, paraoxonase type 1 (PON-1) and glutathione peroxidase (GPx) were determined in serum and CUPRAC, ferric reducing ability of saliva (FRAS) and TEAC in saliva. The oxidant biomarkers included were total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived compounds (d-ROMs), advanced oxidation protein products (AOPP), and thiobarbituric acid reactive substances (TBARS) in serum and AOPP and TBARS in saliva. Results showed a significantly higher TEAC, PON-1, GPx, TOS, POX-Act, and d-ROMs, and a significantly lower AOPP in serum of dogs with hypothyroidism. Meanwhile, significantly lower FRAS and AOPP were observed in saliva of dogs with hypothyroidism. Once salivary concentrations were corrected based on their total protein concentrations, the only analyte showing significant changes was TBARS which was significantly higher in dogs with hypothyroidism. Our results show that dogs with hypothyroidism present alterations in the redox status in both serum and saliva. This study should be considered a preliminary study and further research addressing these changes should be made using larger populations. The online version contains supplementary material available at 10. 1186/s12917-023-03586-4.
Ajuts:	Ministerio de Economía y Competitividad FJC2019-042475-I
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Antioxidants ; Biomarkers ; Dogs ; Hypothyroidism ; Oxidants ; Oxidative stress
Publicat a:	BMC veterinary research, Vol. 19 (february 2023) , ISSN 1746-6148

DOI: 10.1186/s12917-023-03586-4
PMID: 36732758

11 p, 1.7 MB

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