Measurement of oxidative stress index in seminal plasma can predict in vivo fertility of liquid-stored porcine artificial insemination semen doses
Barranco, Isabel (University of Bologna. Department of Veterinary Medical Sciences)
Rubio, Camila Peres (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Tvarijonaviciute, Asta (Universidad de Murcia. Departamento de Medicina y Cirugía Animal)
Rodriguez-Martinez, Heriberto (Linköping University. Department of Biomedical & Clinical Sciences)
Roca, Jordi (Universidad de Murcia. Departamento de Medicina y Cirugía Animal)

Data:	2021
Resum:	The study evaluated the relation between the oxidative stress index (OSI) in porcine seminal plasma (n = 76) with sperm resilience and in vivo fertility (farrowing rate and litter size of 3137 inseminated sows) of liquid-stored artificial insemination (AI) semen doses. The OSI was assessed as the ratio of advanced oxidation protein products to Trolox-equivalent antioxidant capacity, both measured using an automated analyzer. Sperm motility (computer-assisted sperm analyzer) and viability (flow cytometry) were evaluated in semen AI-doses at 0 and 72 h of storage at 17 °C. Sperm resilience was defined as the difference between storage intervals. Semen AI-doses were hierarchically clustered as having high, medium and low seminal OSI (p < 0. 001) with those of low displaying higher resilience (p < 0. 01). Boars were hierarchically clustered into two groups (p < 0. 001) as having either positive or negative farrowing rate and litter size deviation; the negative one showing higher seminal OSI (p < 0. 05). In sum, seminal OSI was negatively related to sperm motility and the in vivo fertility of liquid-stored boar semen AI-doses, with the receiver operating characteristic curve presenting seminal OSI as a good predictive biomarker of in vivo fertility of AI-boars (area under the curve: 0. 815, p < 0. 05).
Ajuts:	Ministerio de Ciencia e Innovación FJC2019-042475-I Ministerio de Economía y Competitividad RYC-2017-22992 European Commission. Horizon 2020 891382
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	In vivo fertility ; Oxidative stress index ; Seminal plasma ; Sperm quality ; Pig
Publicat a:	Antioxidants, Vol. 10 Núm. 8 (august 2021) , p. 1203, ISSN 2076-3921

DOI: 10.3390/antiox10081203
PMID: 34439450

12 p, 1.4 MB

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