Knock-Out of CmNAC-NOR Affects Melon Climacteric Fruit Ripening
Liu, Bin (Centre de Recerca en Agrigenòmica)
Santo Domingo, Miguel (Centre de Recerca en Agrigenòmica)
Mayobre, Carlos (Centre de Recerca en Agrigenòmica)
Martín-Hernández, Ana Montserrat (Centre de Recerca en Agrigenòmica)
Pujol Abajo, Marta (Centre de Recerca en Agrigenòmica)
Garcia-Mas, Jordi (Centre de Recerca en Agrigenòmica)

Date:	2022
Abstract:	Fruit ripening is an important process that affects fruit quality. A QTL in melon, ETHQV6. 3, involved in climacteric ripening regulation, has been found to be encoded by CmNAC-NOR, a homologue of the tomato NOR gene. To further investigate CmNAC-NOR function, we obtained two CRISPR/Cas9-mediated mutants (nor-3 and nor-1) in the climacteric Védrantais background. nor-3, containing a 3-bp deletion altering the NAC domain A, resulted in ~8 days delay in ripening without affecting fruit quality. In contrast, the 1-bp deletion in nor-1 resulted in a fully disrupted NAC domain, which completely blocked climacteric ripening. The nor-1 fruits did not produce ethylene, no abscission layer was formed and there was no external color change. Additionally, volatile components were dramatically altered, seeds were not well developed and flesh firmness was also altered. There was a delay in fruit ripening with the nor-1 allele in heterozygosis of ~20 days. Our results provide new information regarding the function of CmNAC-NOR in melon fruit ripening, suggesting that it is a potential target for modulating shelf life in commercial climacteric melon varieties.
Grants:	Ministerio de Economía y Competitividad AGL2015-64625-C2-1-R Agencia Estatal de Investigación RTI2018-097665-B-C2 Ministerio de Economía y Competitividad SEV-2015-0533 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1319 Agencia Estatal de Investigación BES-2017-079956
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya; FI grant from the Secretaria d'Universitats i Recerca del Departament d'Empresa i Coneixement de la Generalitat de Catalunya; European Social Fund (ESF)
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Fruit ripening ; Melon (Cucumis melo L.) ; NAC-NOR ; CRISPR ; Shelf life
Published in:	Frontiers in plant science, Vol. 13 (June 2022) , art. 878037, ISSN 1664-462X

DOI: 10.3389/fpls.2022.878037
PMID: 35755703

11 p, 2.6 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles