Cellular components and TGF-β1 content of a closed Tube system for Platelet Rich Plasma acquisition in horse
Fantini, Priscila (Universitário UNA)
Jiménez Aristizabal, Román Fulgencio (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Iborra Obiols, Antoni (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Miranda, A.L.S. (Centro Universitário UNA)
Maranhão, R.P.A. (Universidade Federal de Minas Gerais. Escola de Veterinária)
Leme, F.O.P. (Ministry of Education of Brazil)
Palhares, M.S. (Ministry of Education of Brazil)
Prades, Marta (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)

Additional title:	Componentes celulares e TGF-β1 do plasma rico em plaquetas obtido por sistema fechado em equinos
Date:	2022
Abstract:	Platelet-rich plasma (PRP) has been proposed as an agent to accelerate the healing process and stimulate the regenerative capacity of tissues due to its abundance of growth factors. A large variety of kits and protocols are available to obtain PRP by different cell-separation systems. However, the lack of standardization may lead to inconsistent results. The aim of this study was to characterize cellular composition, platelet parameters using the ADVIA 120 flow cytometer, and TGF-β1 concentration from the PRP product obtained through a closed system, using simple centrifugation. Six clinically healthy horses were used in this study. The protocol in the closed system resulted in approximately 1. 6-fold higher platelet and approximately 2. 0-fold lower white blood cell concentrations in comparison with whole blood values. The evaluated system was efficient in concentrating platelets and in retrieving a small number of leukocytes, using a protocol of single centrifugation at low speed.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Portuguès
Document:	Article ; recerca ; Versió publicada
Subject:	PRP ; Growth factors ; Platelet concentrate ; Concentration protocol
Published in:	Arquivo brasileiro de medicina veterinária e zootecnia, Vol. 74 Núm. 1 (2022) , p. 93-100, ISSN 1678-4162

DOI: 10.1590/1678-4162-12257

8 p, 613.1 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Articles > Research articles
Articles > Published articles