Loss-of-function of NITROGEN LIMITATION ADAPTATION confers disease resistance in Arabidopsis by modulating hormone signaling and camalexin content
Val-Torregrosa, Beatriz (Centre de Recerca en Agrigenòmica)
Bundó Barberà, Mireia (Centre de Recerca en Agrigenòmica)
Mallavarapu, Mani Deepika (Centre de Recerca en Agrigenòmica)
Chiou, Tzyy-Jen (Academia Sinica. Agricultural Biotechnology Research Center)
Flors, Victor (Univesitat Jaume I. Departament de Ciències Agràries i del Medi Natural)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)

Date:	2022
Abstract:	Phosphorus is an important macronutrient required for plant growth and development. It is absorbed by the roots in the form of inorganic phosphate (Pi). Under Pi limiting conditions, plants activate the Phosphate Starvation Response (PSR) system to enhance Pi acquisition. The NITROGEN LIMITATION ADAPTION (NLA) gene is a component of the Arabidopsis PSR, and its expression is post-transcriptionally regulated by miR827. We show that loss-of-function of NLA and MIR827 overexpression increases Pi level and enhances resistance to infection by the fungal pathogen Plectosphaerella cucumerina in Arabidopsis. Upon pathogen infection, high Pi plants (e. g. nla plants and wild type plants grown under high Pi supply) showed enhanced callose deposition. High Pi plants also exhibited superinduction of camalexin biosynthesis genes which is consistent with increased levels of camalexin during pathogen infection. Pathogen infection and treatment with fungal elicitors, triggered up-regulation of MIR827 and down-regulation of NLA expression. Under non-infection conditions, the nla plants showed increased levels of SA and JA compared with wild type plants, their levels further increasing upon pathogen infection. Overall, the outcomes of this study suggest that NLA plays a role in Arabidopsis immunity, while supporting convergence between Pi signaling and immune signaling in Arabidopsis.
Grants:	Agencia Estatal de Investigación RTI2018-101275-B-I00 "la Caixa" Foundation LCF/BQ/DI20/11780039 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Ciencia e Innovación CEX2019-000902-S Agencia Estatal de Investigación BES-2016-076289
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya ; Plan de Promoción de la Investigación Universitat Jaume I (UJI-B2019-2) ; "Servicios Centrales de Instrumentación Científica (SCIC), Universitat Jaume I
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Arabidopsis ; Camalexin ; Jasmonic acid ; Phosphate ; Plectosphaerella cucumerina ; Salicylic acid
Published in:	Plant science, Vol. 323 (October 2022) , art. 111374, ISSN 1873-2259

DOI: 10.1016/j.plantsci.2022.111374

12 p, 7.2 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles