Evaluation of a chronic exposure to nanoplastics in goldfish (Carassius auratus): Analytical validation of automated assays for the measurement of biochemical markers
Blonç, Manuel (Universitat Autònoma de Barcelona. Departament de Biologia Cel.lular, de Fisiologia i d'Immunologia)
Brandts, Irene (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Cánovas, M. (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Franco-Martinez, Lorena (Universidad de Murcia)
Rubio, Camila Peres (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel.lular, de Fisiologia i d'Immunologia)
Tvarijonaviciute, Asta (Universidad de Murcia)
Gravato, Carlos (University of Lisbon)
Teles, Mariana (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")

Date:	2023
Abstract:	The bulk of plastic pollution is mainly composed of small fragments, micro- and nanoplastics (NPs). Although many studies are currently published on NPs, research on the effects of NPs in fish after a chronic exposure is still scarce. The present study aimed to validate a series of automated assays to be used in the monitoring of fish challenged with a chronic exposure to NPs, using Carassius auratus (goldfish) as model species. For this purpose, adult C. auratus were exposed to 100 µg/L polystyrene (PS)-NPs for a 30-day period. Total oxidative status (TOS), total antioxidant capacity (TAC), esterase activity (EA) and adenosine deaminase activity (ADA) were measured in the gills, brain and muscle of fish. In addition, acetylcholinesterase activity (AChE) and creatinine kinase (CK) were measured in the muscle. All biomarkers were successfully validated in goldfish tissues and consequently used to assess the effects of NPs following a chronic exposure. Results showed that EA and TAC significantly increased in gills, while EA decreased significantly in the brain, and no effects were observed in any of these parameters in muscle. These results indicate that both immune and antioxidant responses were triggered by NPs in gills, but not in the brain nor muscle. This suggests that gills may be a primary target for NPs, potentially leading to a cascading effect on gas exchange, or osmo- and ionic regulation that should be further investigated.
Grants:	European Commission 956481 Ministerio de Ciencia e Innovación RYC2019-026841-I Agencia Estatal de Investigación PID2020-113221RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2018/FI_B_00711 Ministerio de Economía y Competitividad FJC2019-042475-I
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Automated assay validation ; Biomarkers ; Nanoparticles ; Fish
Published in:	Ecological indicators, Vol. 147 (March 2023) , art. 109966, ISSN 1470-160X

DOI: 10.1016/j.ecolind.2023.109966

8 p, 1.2 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles