Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas
Sprankel, Lasse (Goethe University Frankfurt. Buchmann Institute for Molecular Life Sciences and Institute of Biophysics)
Vizarraga, David (Institut de Biologia Molecular de Barcelona)
Martín, Jesús (Institut de Biologia Molecular de Barcelona)
Manger, Sina (Goethe University Frankfurt. Buchmann Institute for Molecular Life Sciences and Institute of Biophysics)
Meier-Credo, Jakob (Max Planck Institute of Biophysics)
Marcos, Marina (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Julve i Gil, Josep (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Rotllan, Noemi (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Scheffer, Margot P. (Goethe University Frankfurt. Buchmann Institute for Molecular Life Sciences and Institute of Biophysics)
Escolà-Gil, Joan Carles (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Langer, Julian D. (Max Planck Institute for Brain Research)
Piñol Ribas, Jaume (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Fita, Ignacio (Institut de Biologia Molecular de Barcelona)
Frangakis, Achilleas S. (V. Buchmann Institute for Molecular Life Sciences and Institute of Biophysics)

Date:	2023
Abstract:	Mycoplasma pneumoniae, responsible for approximately 30% of community-acquired human pneumonia, needs to extract lipids from the host environment for survival and proliferation. Here, we report a comprehensive structural and functional analysis of the previously uncharacterized protein P116 (MPN_213). Single-particle cryo-electron microscopy of P116 reveals a homodimer presenting a previously unseen fold, forming a huge hydrophobic cavity, which is fully accessible to solvent. Lipidomics analysis shows that P116 specifically extracts lipids such as phosphatidylcholine, sphingomyelin and cholesterol. Structures of different conformational states reveal the mechanism by which lipids are extracted. This finding immediately suggests a way to control Mycoplasma infection by interfering with lipid uptake.
Grants:	Agencia Estatal de Investigación BIO2017-84166-R Agencia Estatal de Investigación PID2021-125632OB-C22 Agencia Estatal de Investigación PID2021-125632OB-C21
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cryoelectron microscopy ; Pathogens ; Structural biology ; Membrane lipids
Published in:	Nature structural & molecular biology, Vol. 30 (February 2023) , p. 321-329, ISSN 1545-9985

DOI: 10.1038/s41594-023-00922-y
PMID: 36782049

28 p, 8.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles