Detection and genetic characterization of enteric viruses in diarrhoea outbreaks from swine farms in Spain
Puente, Héctor (Universidad de León. Departamento de Sanidad Animal)
Arguello, Héctor (Universidad de León. INDEGSAL)
Cortey, Martí (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Gómez-García, Manuel (Universidad de León. Departamento de Sanidad Animal)
Mencía-Ares, Oscar (Universidad de León. Departamento de Sanidad Animal)
Pérez-Perez, Lucía (Universidad de León. Departamento de Sanidad Animal)
Díaz, Ivan (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Carvajal, Ana (Universidad de León. INDEGSAL)

Date:	2023
Abstract:	The aim of this work was to study the prevalence and distribution of Porcine astrovirus (PAstV), Porcine kobuvirus (PKoV), Porcine torovirus (PToV), Mammalian orthoreovirus (MRV) and Porcine mastadenovirus (PAdV) as well as their association with widely recognized virus that cause diarrhoea in swine such as coronavirus (CoVs) and rotavirus (RVs) in diarrhoea outbreaks from Spanish swine farms. Furthermore, a selection of the viral strains was genetically characterized. PAstV, PKoV, PToV, MRV and PAdV were frequently detected. Particularly, PAstV and PKoV were detected in almost 50% and 30% of the investigated farms, respectively, with an age-dependent distribution; PAstV was mainly detected in postweaning and fattening pigs, while PKoV was more frequent in sucking piglets. Viral co-infections were detected in almost half of the outbreaks, combining CoVs, RVs and the viruses studied, with a maximum of 5 different viral species reported in three investigated farms. Using a next generation sequencing approach, we obtained a total of 24 ARN viral genomes (.
Grants:	Ministerio de Ciencia y Tecnología E-RTA2015-0003-C02-02 Ministerio de Ciencia y Tecnología E-RTA2015-0003-C02-01 Ministerio de Ciencia y Tecnología FPU17/00466 Ministerio de Educación, Cultura y Deporte FPU16/03485 Ministerio de Ciencia y Tecnología PRE2020-093762 Ministerio de Ciencia y Tecnología RYC-2015-1715-4 Ministerio de Ciencia y Tecnología BEAGAL-18-106
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Swine ; Multiplex RT-PCR ; Phylogenetic analysis ; Porcine astrovirus ; Porcine kobuvirus ; Porcine torovirus ; Mammalian orthoreovirus ; Porcine mastadenovirus
Published in:	Porcine health management, Vol. 9 (june 2023) , ISSN 2055-5660

DOI: 10.1186/s40813-023-00326-w
PMID: 37349807

14 p, 2.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles