visitant ::
identificació
|
|||||||||||||||
Cerca | Lliura | Ajuda | Servei de Biblioteques | Sobre el DDD | Català English Español |
Pàgina inicial > Articles > Articles publicats > Down-regulation of tomato STEROL GLYCOSYLTRANSFERASE 1 perturbs plant development and facilitates viroid infection |
Data: | 2022 |
Resum: | Potato spindle tuber viroid (PSTVd) is a plant pathogen naturally infecting economically important crops such as tomato (Solanum lycopersicum). Here, we aimed to engineer tomato plants highly resistant to PSTVd and developed several S. lycopersicum lines expressing an artificial microRNA (amiRNA) against PSTVd (amiR-PSTVd). Infectivity assays revealed that amiR-PSTVd-expressing lines were not resistant but instead hypersusceptible to the viroid. A combination of phenotypic, molecular, and metabolic analyses of amiRNA-expressing lines non-inoculated with the viroid revealed that amiR-PSTVd was accidentally silencing the tomato STEROL GLYCOSYLTRANSFERASE 1 (SlSGT1) gene, which caused late developmental and reproductive defects such as leaf epinasty, dwarfism, or reduced fruit size. Importantly, two independent transgenic tomato lines each expressing a different amiRNA specifically designed to target SlSGT1 were also hypersusceptible to PSTVd, thus demonstrating that down-regulation of SlSGT1 was responsible for the viroid-hypersusceptibility phenotype. Our results highlight the role of sterol glycosyltransferases in proper plant development and indicate that the imbalance of sterol glycosylation levels favors viroid infection, most likely by facilitating viroid movement. |
Ajuts: | Agencia Estatal de Investigación RTI2018-095118-A-100 Agencia Estatal de Investigación RYC-2017-21648 Agencia Estatal de Investigación PRE2019-088439 Agencia Estatal de Investigación PIE2020-116765RB-I00 Agencia Estatal de Investigación PID2020-114691RB-I00 Agencia Estatal de Investigación AGL2017-88842-R Agencia Estatal de Investigación CEX2019-000902-S Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-710 |
Drets: | Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Llengua: | Anglès |
Document: | Article ; recerca ; Versió publicada |
Matèria: | Artificial microRNA ; Sterol glycosyltransferase ; Viroid ; Hypersusceptibility ; Solanum lycopersicum |
Publicat a: | Journal of experimental botany, Vol. 74, Issue 5 (March 2022) , p. 1564-1578, ISSN 1460-2431 |
15 p, 4.5 MB |