Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia
Faura, Júlia (Hospital Universitari Vall d'Hebron)
Ramiro, Laura (Hospital Universitari Vall d'Hebron)
Simats Oriol, Alba (Hospital Universitari Vall d'Hebron)
Ma, Feifei (Hospital Universitari Vall d'Hebron)
Penalba, Anna (Hospital Universitari Vall d'Hebron)
Gasull Dalmau, Teresa (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Rosell Novel, Anna (Hospital Universitari Vall d'Hebron)
Montaner, Joan (Hospital Universitari Vall d'Hebron)
Bustamante, Alejandro (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Universitat Autònoma de Barcelona

Date:	2022
Abstract:	After stroke and other brain injuries, there is a high incidence of respiratory complications such as pneumonia or acute lung injury. The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum, brain, and lung homogenate samples 24 h after surgery. A 92 proteins-panel developed by Olink Proteomics ® was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than sham -controls, but these levels did not correlate with the infarct volume. No alteration in alveolar-capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0. 1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-α, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA. This study confirmed that post-stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-α, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke-induced lung damage.
Grants:	Instituto de Salud Carlos III PI17/02130 Instituto de Salud Carlos III PI21/00939 Instituto de Salud Carlos III PI17/02130 Instituto de Salud Carlos III PI21/00939
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Inflammation ; Pulmonary complications ; Stroke
Published in:	International journal of molecular sciences, Vol. 23 (july 2022) , ISSN 1422-0067

DOI: 10.3390/ijms23158093
PMID: 35897671

12 p, 1.2 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles