Detection of cytomegalovirus in bronchoalveolar lavage fluid from immunocompromised patients with pneumonitis by viral culture and DNA quantification
Berengua, Carla (Institut d'Investigació Biomèdica Sant Pau)
Miró, Elisenda (Institut d'Investigació Biomèdica Sant Pau)
Gutiérrez, C. (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Sánchez, M. (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Mulero, A. (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Ramos, P. (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Del Cuerpo, Margarita (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Torrego, A. (Institut d'Investigació Biomèdica Sant Pau)
García-Cadenas, I. (Institut d'Investigació Biomèdica Sant Pau)
Pajares Ruiz, Virginia (Institut d'Investigació Biomèdica Sant Pau)
Navarro Risueño, Ferran (Institut d'Investigació Biomèdica Sant Pau)
Martino Bofarull, Rodrigo (Institut d'Investigació Biomèdica Sant Pau)
Rabella, Núria (Institut d'Investigació Biomèdica Sant Pau)
Universitat Autònoma de Barcelona

Date:	2023
Description:	6 pàg.
Abstract:	To compare the detection of human cytomegalovirus (HCMV) in bronchoalveolar lavage (BAL) fluid by viral culture and quantitative polymerase chain reaction (qPCR), and to establish a viral load threshold that can identify cases of HCMV replication indicative of pneumonitis. There is currently no universal viral load cut-off to differentiate between patients with and without pneumonitis, and the interpretation of qPCR results is challenging. 176 consecutive BAL samples from immunosuppressed hosts with signs and/or symptoms of respiratory infection were prospectively studied by viral culture and qPCR. Concordant results were obtained in 81. 25% of the BAL samples. The rest were discordant, as only 34% of the qPCR-positive BAL samples were positive by culture. The median HCMV load was significantly higher in culture-positive than in culture-negative BAL samples (5038 vs 178 IU/mL). Using a cut-off value of 1258 IU/mL of HCMV in BAL, pneumonia was diagnosed with a sensitivity of 76%, a specificity of 100%, a VPP of 100% and VPN of 98%, and HCMV was isolated in 100% of the BAL cultures. We found that a qPCR-negative was a quick and reliable way of ruling out HCMV pneumonitis, but a positive result did not always indicate clinically significant replication in the lung. However, an HCMV load in BAL fluid of ≥ 1258 IU/mL was always associated with disease, whereas < 200 IU/mL rarely so.
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Cytomegalovirus ; HCMV ; Immunosuppressed ; Pneumonitis ; Quantitative PCR ; Viral culture ; Viral load
Published in:	Journal of Virological Methods, Vol. 317 (2023) , p. 114743, ISSN 1879-0984

DOI: 10.1016/j.jviromet.2023.114743
PMID: 37116585

6 p, 977.9 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles