Porcine Reproductive and Respiratory Syndrome (PRRSV2) Viral Diversity within a Farrow-to-Wean Farm Cohort Study
Kikuti, Mariana (University of Minnesota. Department of Veterinary Population Medicine)
Vilalta, Carles (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Sanhueza, Juan (Universidad Católica de Temuco. Departamento de Ciencias Veterinarias y Salud Pública)
Pamornchainavakul, Nakarin (University of Minnesota. Department of Veterinary Population Medicine)
Kevill, Jessica (Bangor University. Centre for Environmental Biotechnology)
Yang, My (University of Minnesota. Department of Veterinary Population Medicine)
Paploski, Igor A. D. (University of Minnesota. Department of Veterinary Population Medicine)
Lenskaia, Tatiana (University of Minnesota. Department of Veterinary Population Medicine)
Odogwu, Nkechi M. (University of Minnesota. Department of Veterinary Population Medicine)
Kiehne, Ross (Swine Vet Center)
VanderWaal, Kimberly (University of Minnesota. Department of Veterinary Population Medicine)
Schroeder, Declan (University of Minnesota. Department of Veterinary Population Medicine)
Corzo, Cesar A. (University of Minnesota. Department of Veterinary Population Medicine)

Data:	2023
Resum:	Describing PRRSV whole-genome viral diversity data over time within the host and within-farm is crucial for a better understanding of viral evolution and its implications. A cohort study was conducted at one naïve farrow-to-wean farm reporting a PRRSV outbreak. All piglets 3-5 days of age (DOA) born to mass-exposed sows through live virus inoculation with the recently introduced wild-type virus two weeks prior were sampled and followed up at 17-19 DOA. Samples from 127 piglets were individually tested for PRRSV by RT-PCR and 100 sequences were generated using Oxford Nanopore Technologies chemistry. Female piglets had significantly higher median Ct values than males (15. 5 vs. 13. 7, Kruskal-Wallis p < 0. 001) at 3-5 DOA. A 52. 8% mortality between sampling points was found, and the odds of dying by 17-19 DOA decreased with every one unit increase in Ct values at 3-5 DOA (OR = 0. 76, 95% CI 0. 61-0. 94, p = 0. 01). Although the within-pig percent nucleotide identity was overall high (99. 7%) between 3-5 DOA and 17-19 DOA samples, ORFs 4 and 5a showed much lower identities (97. 26% and 98. 53%, respectively). When looking solely at ORF5, 62% of the sequences were identical to the 3-5 DOA consensus. Ten and eight regions showed increased nucleotide and amino acid genetic diversity, respectively, all found throughout ORFs 2a/2b, 4, 5a/5, 6, and 7.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Porcine reproductive and respiratory syndrome ; Genetic variation ; Whole-genome sequencing ; Molecular epidemiology
Publicat a:	Viruses, Vol. 15 (august 2023) , ISSN 1999-4915

DOI: 10.3390/v15091837
PMID: 37766244

11 p, 2.4 MB

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