Efficacy of the filmarray blood culture identification panel for direct molecular diagnosis of infectious diseases from samples other than blood
Micó, Miquel (Institut d'Investigació Biomèdica Sant Pau)
Navarro Risueño, Ferran (Institut d'Investigació Biomèdica Sant Pau)
de Miniac, Daniela (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
González, Yésica (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Brell, Albert (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
López, Cristina (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Sanchez-Reus, Ferran (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Mirelis, Beatriz (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Coll Figa, Pedro (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2015
Abstract:	Molecular-based techniques reduce the delay in diagnosing infectious diseases and therefore contribute to better patient outcomes. We assessed the FilmArray blood culture identification (BCID) panel (Biofire Diagnostics/bioMérieux) directly on clinical specimens other than blood: cerebrospinal, joint, pleural and ascitic fluids, bronchoscopy samples and abscesses. We compared the results from 88 samples obtained by culture-based techniques. The percentage of agreement between the two methods was 75 % with a Cohen K value of 0. 51. Global sensitivity and specificity using the FilmArray BCID panel were 71 and 97 %, respectively. Sensitivity was poorer in samples with a low bacterial load, such as ascitic and pleural fluids (25 %), whereas the sensitivity for abscess samples was high (89 %). These findings suggest that the FilmArray BCID panel could be useful to perform microbiological diagnosis directly from samples other than positive blood cultures, as it offers acceptable sensitivity and moderate agreement with conventional microbiological methods. Nevertheless, cost-benefit studies should be performed before introducing this method into algorithms for microbiological diagnostics.
Grants:	Instituto de Salud Carlos III REIPI/RD06/0008/0013
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	SDG 3 - Good Health and Well-being
Published in:	Journal of Medical Microbiology, Vol. 64, Issue 12 (December 2015) , p. 1481-1488, ISSN 0022-2615

DOI: 10.1099/jmm.0.000180

Postprint 25 p, 1.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
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