The E1a Adenoviral Gene Upregulates the Yamanaka Factors to Induce Partial Cellular Reprogramming
Mendoza, Gracia (Instituto de Investigación Sanitaria de Aragón)
González-Pastor, Rebeca (Universidad UTE. Centro de Investigación Biomédica)
Sánchez, Juan Miguel (Imperial College London. National Heart and Lung Institute)
Arce Cerezo, Altamira (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Quintanilla, Miguel (Universidad Autónoma de Madrid. Departamento de Bioquímica)
Moreno-Bueno, Gema (Instituto de Salud Carlos III. Centro de Investigación Biomédica en Red)
Pujol i Altarriba, Anna (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Belmar-López, Carolina (Universidad Privada San Juan Bautista)
de Martino, Alba (Instituto de Investigación Sanitaria de Aragón)
Riu, Efrén (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Rodriguez, Tristan A. (Imperial College London. National Heart and Lung Institute)
Martin-Duque, Pilar (Universidad de Zaragoza. Departamento de Cirugía)
Date: |
2023 |
Abstract: |
The induction of pluripotency by enforced expression of different sets of genes in somatic cells has been achieved with reprogramming technologies first described by Yamanaka's group. Methodologies for generating induced pluripotent stem cells are as varied as the combinations of genes used. It has previously been reported that the adenoviral E1a gene can induce the expression of two of the Yamanaka factors (c-Myc and Oct-4) and epigenetic changes. Here, we demonstrate that the E1a-12S over-expression is sufficient to induce pluripotent-like characteristics closely to epiblast stem cells in mouse embryonic fibroblasts through the activation of the pluripotency gene regulatory network. These findings provide not only empirical evidence that the expression of one single factor is sufficient for partial reprogramming but also a potential mechanistic explanation for how viral infection could lead to neoplasia if they are surrounded by the appropriate environment or the right medium, as happens with the tumorogenic niche. |
Grants: |
Instituto de Salud Carlos III PI19/01007 Instituto de Salud Carlos III DTS21/00130
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Rights: |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
Language: |
Anglès |
Document: |
Article ; recerca ; Versió publicada |
Subject: |
Reprogramming ;
Induced pluripotent stem cells ;
Embryonic stem cells ;
Yamanaka factors ;
Adenovirus ;
E1a gene |
Published in: |
Cells, Vol. 12 (may 2023) , ISSN 2073-4409 |
DOI: 10.3390/cells12091338
PMID: 37174738
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Record created 2023-12-02, last modified 2024-02-27