Myocardium of patients with dilated cardiomyopathy presents altered expression of genes involved in thyroid hormone biosynthesis
Gil-Cayuela, Carolina (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Ortega, Ana (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Tarazón, Estefanía (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Martínez-Dolz, Luis (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Cinca, Juan (Institut d'Investigació Biomèdica Sant Pau)
González-Juanatey, José Ramón (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Lago, Francisca (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Roselló-Lletí, Esther (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Rivera, Miguel (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Portolés, Manuel (Centro de Investigación Biomédica en Red en Enfermedades Cardiovasculares)
Universitat Autònoma de Barcelona

Date:	2018
Abstract:	The association between dilated cardiomyopathy (DCM) and low thyroid hormone (TH) levels has been previously described. In these patients abnormal thyroid function is significantly related to impaired left ventricular (LV) function and increased risk of death. Although TH was originally thought to be produced exclusively by the thyroid gland, we recently reported TH biosynthesis in the human ischemic heart. Based on these findings, we evaluated whether the genes required for TH production are also altered in patients with DCM. Methods Twenty-three LV tissue samples were obtained from patients with DCM (n = 13) undergoing heart transplantation and control donors (n = 10), and used for RNA sequencing analysis. The number of LV DCM samples was increased to 23 to determine total T4 and T3 tissue levels by ELISA. We found that all components of TH biosynthesis are expressed in human dilated heart tissue. Expression of genes encoding thyroperoxidase (-2. 57-fold, P < 0. 05) and dual oxidase 2 (2. 64-fold, P < 0. 01), the main enzymatic system of TH production, was significantly altered in patients with DCM and significantly associated with LV remodeling parameters. Thyroxine (T4) cardiac tissue levels were significantly increased (P < 0. 01), whilst triiodothyronine (T3) levels were significantly diminished (P < 0. 05) in the patients. Expression of TH biosynthesis machinery in the heart and total tissue levels of T4 and T3, are altered in patients with DCM. Given the relevance of TH in cardiac pathology, our results provide a basis for new gene-based therapeutic strategies for treating DCM.
Grants:	Ministerio de Economía y Competitividad PI13/00100 Ministerio de Economía y Competitividad PI14/01506 Ministerio de Economía y Competitividad CB16/11/00261
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PloS one, Vol. 13 Núm. 1 (january 2018) , p. e0190987, ISSN 1932-6203

DOI: 10.1371/journal.pone.0190987
PMID: 29320567

12 p, 2.0 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles