Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
Rodríguez-Vázquez, Lucía (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Martí-Clúa, Joaquín (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)

Data:	2021
Descripció:	29 pàg.
Resum:	The current study was conducted to assess whether a single administration of 5-bromo-2'-deoxyuridine (BrdU) interferes with cell proliferation and leads to the activation of apoptotic cellular events in the prenatal cerebellum. BrdU effects across a wide range of doses (25-300 mu g/g b. w. ) were analyzed using immunohistochemical and ultrastructural procedures. The pregnant rats were injected with BrdU at embryonic day 13, and their fetuses were sacrificed from 5 to 35 hr after exposure. The quantification of several parameters such as the density of mitotic figures, and BrdU and proliferating cell nuclear antigen (PCNA)-reactive cells showed that, in comparison with the saline injected rats, the administration of BrdU impairs the proliferative behavior of neuroepithelial cells. The above-mentioned parameters were significantly reduced in rats injected with 100 mu g/g b. w. of BrdU. The reduction was more evident using 200 mu g/g b. w. The most severe effects were found with 300 mu g/g b. w. of BrdU. The present findings also revealed that high doses of BrdU lead to the activation of apoptotic cellular events as evidenced by both terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and immunohistochemistry for active caspase-3. In comparison with saline rats, many apoptotic cells were found in rats injected with 100 mu g/g b. w. of BrdU. The number of dying cells increased with 200 mu g/g b. w. The most important number of apoptotic cells were observed in animals injected with 300 mu g/g b. w. of BrdU. Ultrastructural studies confirmed the presence of neuroblasts at different stages of apoptosis.
Drets:	Tots els drets reservats.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	5-bromo-2'-deoxyuridine ; RRID: AB_10013660 ; RRID: AB_2313609 ; RRID: AB_258588 ; RRID: AB_476884 ; RRID: AB_628110 ; Apoptosis ; Cerebellar neuroepithelium ; Embryonic life ; Immunohistochemistry ; Transmission electron microscopy
Publicat a:	Journal of Comparative Neurology, Vol. 529 Núm. 5 (2021) , ISSN 1096-9861

DOI: 10.1002/cne.25005
PMID: 32785933

29 p, 233.1 KB

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