(Department of Immunology and Oncology and Nanobiomedicine Initiative. Centro Nacional de Biotecnología (CNB-CSIC))
(Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
(Department of Immunology and Oncology and Nanobiomedicine Initiative. Centro Nacional de Biotecnología (CNB-CSIC))
| Data: |
2023 |
| Resum: |
Upon contact with biological fluids like serum, a protein corona (PC) complex forms on iron oxide nanoparticles (IONPs) in physiological environments and the proteins it contains influence how IONPs act in biological systems. Although the biological identity of PC-IONP complexes has often been studied in vitro and in vivo, there have been inconsistent results due to the differences in the animal of origin, the type of biological fluid, and the physicochemical properties of the IONPs. Here, we identified differences in the PC composition when it was derived from the sera of three species (bovine, murine, or human) and deposited on IONPs with similar core diameters but with different coatings [dimercaptosuccinic acid (DMSA), dextran (DEX), or 3-aminopropyl triethoxysilane (APS)], and we assessed how these differences influenced their effects on macrophages. We performed a comparative proteomic analysis to identify common proteins from the three sera that adsorb to each IONP coating and the 10 most strongly represented proteins in PCs. We demonstrated that the PC composition is dependent on the origin of the serum rather than the nature of the coating. The PC composition critically affects the interaction of IONPs with macrophages in self- or non-self identity models, influencing the activation and polarization of macrophages. However, such effects were more consistent for DMSA-IONPs. As such, a self biological identity of IONPs promotes the activation and M2 polarization of murine macrophages, while a non-self biological identity favors M1 polarization, producing larger quantities of ROS. In a human context, we observed the opposite effect, whereby a self biological identity of DMSA-IONPs promotes a mixed M1/M2 polarization with an increase in ROS production. Conversely, a non-self biological identity of IONPs provides nanoparticles with a stealthy character as no clear effects on human macrophages were evident. Thus, the biological identity of IONPs profoundly affects their interaction with macrophages, ultimately defining their biological impact on the immune system. |
| Ajuts: |
Agencia Estatal de Investigación SAF2017-82223-R
Ministerio de Ciencia e Innovación 10.13039/501100011033
|
| Nota: |
Fundació Carreras |
| Nota: |
The authors acknowledge the Scientific and Technical Assistance of the Proteomics, Transmission electron microscopy, Flow cytometry, and Confocal microscopy facilities at the CNB. ICP-OES analysis was carried out in the support laboratories of Instituto de Ciencia de Materiales de Madrid (CSIC). The authors are also grateful to M. Sefton for the author editing of the manuscript. This work was supported by the following grants: Grant SAF2017-82223-R (to D.F.B.) funded by MCIN/AEI/10.13039/501100011033; and an ERDF a way of making Europe and Grant PID2020-112685RB-100 (to D.F.B.) funded by the MCIN/AEI/10.13039/501100011033. V.M.-A. was a postdoctoral scholar working under a Juan de La Cierva-Incorporación Contract (IJCI-2017-31447, funded by MCIN/AEI/10.13039/501100011033), and Y. P. was first a predoctoral FPU scholar (FPU15/06170) funded by MCIN/AEI/10.13039/501100011033 and by "ESF Investing in your future", then a predoctoral scholar funded by CSIC-COV19-012/012202020E154 intramural project and finally a postdoctoral scholar funded by the European Commission-NextGenerationEU (Regulation EU2020/2094) through the CSIC's Global Health Platform (PTI Salud Global, SGL2103021). This research work was performed in the framework of the Nanomedicine CSIC HUB (ref 202180E048). |
| Nota: |
This work was supported by the following grants: Grant SAF2017-82223-R (to D.F.B.) funded by MCIN/AEI/10.13039/501100011033; and an ERDF a way of making Europe and Grant PID2020-112685RB-100 (to D.F.B.) funded by the MCIN/AEI/10.13039/501100011033. V.M.-A. was a postdoctoral scholar working under a Juan de La Cierva-Incorporación Contract (IJCI-2017-31447, funded by MCIN/AEI/10.13039/501100011033), and Y. P. was first a predoctoral FPU scholar (FPU15/06170) funded by MCIN/AEI/10.13039/501100011033 and by "ESF Investing in your future", then a predoctoral scholar funded by CSIC-COV19-012/012202020E154 intramural project and finally a postdoctoral scholar funded by the European Commission-NextGenerationEU (Regulation EU2020/2094) through the CSIC's Global Health Platform (PTI Salud Global, SGL2103021). This research work was performed in the framework of the Nanomedicine CSIC HUB (ref 202180E048). |
| Drets: |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.  |
| Llengua: |
Anglès |
| Document: |
Article ; recerca ; Versió publicada |
| Matèria: |
Magnetic nanoparticles ;
Protein corona ;
Mouse serum ;
Human serum ;
Macrophage activation |
| Publicat a: |
ACS applied materials & interfaces, Vol. 15 Núm. 30 (february 2023) , p. 35906-35926, ISSN 1944-8252 |
visitant ::
identificació
(Department of Immunology and Oncology and Nanobiomedicine Initiative. Centro Nacional de Biotecnología (CNB-CSIC))
