Adipocyte fatty-acid binding protein is overexpressed in cirrhosis and correlates with clinical outcomes
Graupera, Isabel (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Coll, Mar (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Pose, Elisa (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Elia, Chiara (Hospital Clínic i Provincial de Barcelona)
Piano, Salvatore (Hospital Clínic i Provincial de Barcelona)
Solà, Elsa (Hospital Clínic i Provincial de Barcelona)
Blaya, Delia (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Huelin, Patricia (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Solé, Cristina (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Moreira, Rebeca (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
De Prada, Gloria (Hospital Clínic i Provincial de Barcelona)
Fabrellas, Núria (Institut d'Investigacions Biomèdiques August Pi i Sunyer)
Juanola Mayos, Adrià (Hospital Clínic i Provincial de Barcelona)
Morales Ruiz, Manuel (Hospital Clínic i Provincial de Barcelona)
Sancho-Bru, Pau (Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas)
Villanueva, Càndid (Institut d'Investigació Biomèdica Sant Pau)
Ginès, Pere (Hospital Clínic i Provincial de Barcelona)
Universitat Autònoma de Barcelona

Date:	2017
Abstract:	Fatty-acid-binding proteins (FABPs) are small intracellular proteins that coordinate lipid-mediated processes by targeting metabolic and immune response pathways. The aim of the study was to investigate plasma FABPs levels and their relationship with clinical outcomes in cirrhosis. Plasma levels of L-FABP1(liver and kidney), I-FABP2(intestine), and A-FABP4(adipocyte and macrophages) were measured in 274 patients with decompensated cirrhosis. Hepatic gene expression of FABPs was assessed in liver biopsies from patients with decompensated cirrhosis and in liver cell types from mice with cirrhosis. Immunohistochemistry of A-FABP4 in human liver biopsy was also performed. Plasma levels of FABPs were increased in patients with decompensated cirrhosis compared to those of healthy subjects (L-FABP1: 25 (17-39) vs 10 (9-17) ng/mL p = 0. 001, I-FABP2: 1. 1 (0. 5-2. 1) vs 0. 6 (0. 4-1) ng/mL p = 0. 04 and A-FABP4: 37 (20-68) vs 16 (11-33) ng/mL p = 0. 002), respectively. Increased A-FABP4 levels were associated with complications of cirrhosis, acute-on-chronic liver failure and poor survival. Hepatic A-FABP4 gene expression was upregulated in decompensated cirrhosis. Macrophages were the main liver cell that over-expressed A-FABP4 in experimental cirrhosis and increased A-FABP4 was found in macrophages of human biopsies by immunohistochemistry. A-FABP4 levels are increased in decompensated cirrhosis and correlate with poor outcomes. Liver macrophages appear to be the main source of A-FABP4 in decompensated cirrhosis.
Grants:	Ministerio de Economía y Competitividad PI12/00330 Ministerio de Economía y Competitividad PI16/00043 Ministerio de Economía y Competitividad CM14/00122 Ministerio de Economía y Competitividad FI14/00227
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 7 Núm. 1 (january 2017) , p. 1829, ISSN 2045-2322

DOI: 10.1038/s41598-017-01709-0
PMID: 28500294

11 p, 1.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles