Biomarkers Found in the Tumor Interstitial Fluid may Help Explain the Differential Behavior Among Keratinocyte Carcinomas
Matas-Nadal, Clara (Hospital de Santa Caterina (Salt, Girona))
Bech-Serra, Joan Josep (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Gatius, Sònia (Hospital Universitari Arnau de Vilanova)
Gomez, Xavier (Universitat de Lleida. Departament de Ciències Mèdiques Bàsiques)
Ribes-Santolaria, Marina (Universitat de Lleida. Departament de Ciències Mèdiques Bàsiques)
Guasch-Vallés, Marta (Universitat de Lleida. Departament de Ciències Mèdiques Bàsiques)
Pedraza, Neus (Universitat de Lleida. Departament de Ciències Mèdiques Bàsiques)
Casanova, Josep M. (Hospital Universitari Arnau de Vilanova)
De La Torre Gómez, Gisela Carolina (Institut Germans Trias i Pujol. Institut de Recerca contra la Leucèmia Josep Carreras)
Gari, Eloi (Universitat de Lleida. Departament de Ciències Mèdiques Bàsiques)
Aguayo-Ortiz, Rafael S. (Hospital Universitari Arnau de Vilanova)

Data:	2023
Resum:	Basal cell carcinomas (BCCs) and cutaneous squamous cell carcinomas (SCCs) are the most frequent types of cancer, and both originate from the keratinocyte transformation, giving rise to the group of tumors called keratinocyte carcinomas (KCs). The invasive behavior is different in each group of KC and may be influenced by their tumor microenvironment. The principal aim of the study is to characterize the protein profile of the tumor interstitial fluid (TIF) of KC to evaluate changes in the microenvironment that could be associated with their different invasive and metastatic capabilities. We obtained TIF from 27 skin biopsies and conducted a label-free quantitative proteomic analysis comparing seven BCCs, 16 SCCs, and four normal skins. A total of 2945 proteins were identified, 511 of them quantified in more than half of the samples of each tumoral type. The proteomic analysis revealed differentially expressed TIF proteins that could explain the different metastatic behavior in both KCs. In detail, the SCC samples disclosed an enrichment of proteins related to cytoskeleton, such as Stratafin and Ladinin-1. Previous studies found their upregulation positively correlated with tumor progression. Furthermore, the TIF of SCC samples was enriched with the cytokines S100A8/S100A9. These cytokines influence the metastatic output in other tumors through the activation of NF-kB signaling. According to this, we observed a significant increase in nuclear NF-kB subunit p65 in SCCs but not in BCCs. In addition, the TIF of both tumors was enriched with proteins involved in the immune response, highlighting the relevance of this process in the composition of the tumor environment. Thus, the comparison of the TIF composition of both KCs provides the discovery of a new set of differential biomarkers. Among them, secreted cytokines such as S100A9 may help explain the higher aggressiveness of SCCs, while Cornulin is a specific biomarker for BCCs. Finally, the proteomic landscape of TIF provides key information on tumor growth and metastasis, which can contribute to the identification of clinically applicable biomarkers that may be used in the diagnosis of KC, as well as therapeutic targets.
Ajuts:	Generalitat de Catalunya 2017-SGR-569 Ministerio de Economía y Competitividad PT13/0001 Agencia Estatal de Investigación PID2019-104859GB-I00
Nota:	Altres ajuts: This work was funded by the Spanish Ministry of Innovation and Science MICINN (PID2019-104859GB-I00) and by Generalitat de Catalunya (2017-SGR-569). This work was supported by IRBLleida Biobank (B.0000682) and PLATAFORMA BIOBANCOS PT20/0021. "The proteomics analyses were performed in the IJC Proteomics Unit. The IJC Proteomics Unit is part of the Spanish Platform of Molecular and Bioinformatics Resources (ProteoRed), Instituto de Salud Carlos III (PT13/0001)." For detailed "Experimental procedures" for publication, please, contact the Proteomics Unit. Special thanks to Eddie Chalecki lluMme. M. R.-S. is the recipient of a TALENT grant from Lleida Institute for Biomedical Research-Dr Pifarré Foundation supported by Diputació de Lleida. M. G.-V. (FPU17/00229) was supported by a predoctoral fellowship from Ministerio de Educación, Cultura y Deportes.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Biomarkers, Tumor ; Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Extracellular Fluid ; Humans ; Keratinocytes ; NF-kappa B ; Proteomics ; Skin Neoplasms ; Tumor Microenvironment
Publicat a:	Molecular and Cellular Proteomics, Vol. 22 Núm. 6 (june 2023) , p. 100547, ISSN 1535-9484

DOI: 10.1016/j.mcpro.2023.100547
PMID: 37059366

16 p, 3.5 MB

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