Reduced graphene oxide electrodes meet lateral flow assays : A promising path to advanced point-of-care diagnostics
Calucho, Enric (Universitat Autònoma de Barcelona)
Álvarez Diduk, Ruslan (Institut Català de Nanociència i Nanotecnologia)
Piper, Andrew (Institut Català de Nanociència i Nanotecnologia)
Rossetti, Marianna (Institut Català de Nanociència i Nanotecnologia)
Nevanen, Tarja K. (VTT Technical Research Centre of Finland Ltd)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Date:	2024
Abstract:	Research in electrochemical detection in lateral flow assays (LFAs) has gained significant momentum in recent years. The primary impetus for this surge in interest is the pursuit of achieving lower limits of detection, especially given that LFAs are the most widely employed point-of-care biosensors. Conventionally, the strategy for merging electrochemistry and LFAs has centered on the superposition of screen-printed electrodes onto nitrocellulose substrates during LFA fabrication. Nevertheless, this approach poses substantial limitations regarding scalability. In response, we have developed a novel method for the complete integration of reduced graphene oxide (rGO) electrodes into LFA strips. We employed a CO laser to concurrently reduce graphene oxide and pattern nitrocellulose, exposing its backing to create connection sites impervious to sample leakage. Subsequently, rGO and nitrocellulose were juxtaposed and introduced into a roll-to-roll system using a wax printer. The exerted pressure facilitated the transfer of rGO onto the nitrocellulose. We systematically evaluated several electrochemical strategies to harness the synergy between rGO and LFAs. While certain challenges persist, our rGO transfer technology presents compelling potential for setting a new standard in electrochemical LFA fabrication.
Grants:	Agencia Estatal de Investigación PID2021-124795NB-I00 Agencia Estatal de Investigación PRE2018-084856 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-01464 Agencia Estatal de Investigación CEX2021-001214-S European Commission 881603 European Commission 101029884 European Commission 101120706
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Electrochemistry ; Lateral flow assays ; Laser reduced graphene oxide ; Scalable fabrication ; Nanoflowers
Published in:	Biosensors & bioelectronics, Vol. 258 (August 2024) , art. 116315, ISSN 1873-4235

DOI: 10.1016/j.bios.2024.116315

10 p, 6.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles