Effects of Glucosinolate-Enriched Red Radish (Raphanus sativus) on In Vitro Models of Intestinal Microbiota and Metabolic Syndrome-Related Functionalities
Rosés, Carles (Universitat Autònoma de Barcelona. Servei de Genòmica)
Garcia-Ibañez, Paula (Centro de Edafología y Biología Aplicada del Segura)
Agudelo, Agatha (Universitat Politècnica de València)
Viadel, Blanca (AINIA Technology Centre)
Tomás-Cobos, Lidia (AINIA Technology Centre)
Gallego, Elisa (AINIA Technology Centre)
Carvajal, Micaela (Centro de Edafología y Biología Aplicada del Segura)
Milagro, Fermin I. (Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición)
Barceló Vernet, Anna (Universitat Autònoma de Barcelona. Servei de Genòmica)

Data:	2023
Resum:	The gut microbiota profile is determined by diet composition, and therefore this interaction is crucial for promoting specific bacterial growth and enhancing the health status. Red radish (Raphanus sativus L. ) contains several secondary plant metabolites that can exert a protective effect on human health. Recent studies have shown that radish leaves have a higher content of major nutrients, minerals, and fiber than roots, and they have garnered attention as a healthy food or supplement. Therefore, the consumption of the whole plant should be considered, as its nutritional value may be of greater interest. The aim of this work is to evaluate the effects of glucosinolate (GSL)-enriched radish with elicitors on the intestinal microbiota and metabolic syndrome-related functionalities by using an in vitro dynamic gastrointestinal system and several cellular models developed to study the GSL impact on different health indicators such as blood pressure, cholesterol metabolism, insulin resistance, adipogenesis, and reactive oxygen species (ROS). The treatment with red radish had an influence on short-chain fatty acids (SCFA) production, especially on acetic and propionic acid and many butyrate-producing bacteria, suggesting that consumption of the entire red radish plant (leaves and roots) could modify the human gut microbiota profile toward a healthier one. The evaluation of the metabolic syndrome-related functionalities showed a significant decrease in the gene expression of endothelin, interleukin IL-6, and cholesterol transporter-associated biomarkers (ABCA1 and ABCG5), suggesting an improvement of three risk factors associated with metabolic syndrome. The results support the idea that the use of elicitors on red radish crops and its further consumption (the entire plant) may contribute to improving the general health status and gut microbiota profile.
Nota:	Altres ajuts: The Ministerio de Ciencia e Innovación Centro para el Desarrollo Tecnológico Industrial (CDTI) was the financer of this research: BIOTAGUT (CDTI strategical project 2017)
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	ACS omega, Vol. 8, Issue 26 (June 2023) , p. 23373-23388, ISSN 2470-1343

DOI: 10.1021/acsomega.2c08128
PMID: 37426251

16 p, 8.0 MB

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