Characterization of the molecular mechanisms of silicon uptake in coccolithophores
Ratcliffe, Sarah (University of Bristol)
Meyer, Erin M. (University of North Carolina Wilmington. Department of Biology and Marine Biology)
Walker, Charlotte E. (Marine Biological Association of the United Kingdom)
Knight, Michael (University of Southampton)
McNair, Heather M. (University of California, Santa Barbara. Department of Ecology Evolution and Marine Biology)
Matson, Paul G. (University of California, Santa Barbara. Department of Ecology Evolution and Marine Biology)
Iglesias-Rodriguez, Debora (University of California, Santa Barbara. Department of Ecology Evolution and Marine Biology)
Brzezinski, Mark (University of California, Santa Barbara. Department of Ecology Evolution and Marine Biology)
Langer, Gerald (Marine Biological Association of the United Kingdom)
Sadekov, Aleksey (University of Western Australia)
Greaves, Mervyn (University of Cambridge. Department of Earth Sciences)
Brownlee, Colin (Marine Biological Association of the United Kingdom)
Curnow, Paul (University of Bristol)
Taylor, Alison R. (University of North Carolina Wilmington. Department of Biology and Marine Biology)
Wheeler, Glen (Marine Biological Association of the United Kingdom)

Data:	2023
Resum:	Coccolithophores are an important group of calcifying marine phytoplankton. Although coccolithophores are not silicified, some species exhibit a requirement for Si in the calcification process. These species also possess a novel protein (SITL) that resembles the SIT family of Si transporters found in diatoms. However, the nature of Si transport in coccolithophores is not yet known, making it difficult to determine the wider role of Si in coccolithophore biology. Here, we show that coccolithophore SITLs act as Na+-coupled Si transporters when expressed in heterologous systems and exhibit similar characteristics to diatom SITs. We find that CbSITL from Coccolithus braarudii is transcriptionally regulated by Si availability and is expressed in environmental coccolithophore populations. However, the Si requirement of C. braarudii and other coccolithophores is very low, with transport rates of exogenous Si below the level of detection in sensitive assays of Si transport. As coccoliths contain only low levels of Si, we propose that Si acts to support the calcification process, rather than forming a structural component of the coccolith itself. Si is therefore acting as a micronutrient in coccolithophores and natural populations are only likely to experience Si limitation in circumstances where dissolved silicon (DSi) is depleted to extreme levels.
Ajuts:	European Commission 670390 European Commission 20101109
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Environmental Microbiology, Vol. 25, Issue 2 (February 2023) , p. 315-330, ISSN 1462-2920

DOI: 10.1111/1462-2920.16280
PMID: 36397254

16 p, 1.3 MB

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