Are Hamstring Grafts a Predisposing Factor to Infection in R-ACL Surgery? A Comparative In Vitro Study
Corcoll, Ferran (Hospital del Mar (Barcelona, Catalunya))
Pérez-Prieto, Daniel (Hospital del Mar (Barcelona, Catalunya))
Karbysheva, Svetlana (Charité-Universitätsmedizin Berlin)
Trampuz, Andrej (Charité-Universitätsmedizin Berlin)
Fariñas Barbera, Oscar (Institut Germans Trias i Pujol. Banc de Sang i Teixits)
Monllau García, Joan Carles (Universitat Autònoma de Barcelona. Departament de Ciències Morfològiques)

Data:	2023
Resum:	Background: The objective of the present study was to evaluate the formation of biofilms in bone patellar tendon bone grafts (BPTB grafts), and to compare it to the formation of biofilm formation in quadrupled hamstring anterior cruciate ligament grafts (4×Ht graft). Methods: A descriptive in vitro study was conducted. One 4×Ht graft and one BPTB graft were prepared. They were then contaminated with a strain of S. epidermidis. Later, a quantitative analysis was conducted by means of microcalorimetry and sonication with plating. Additionally, a qualitative analysis was conducted by means of electron microscopy. Results: No significant differences were found between the bacterial growth profiles of the 4×Ht graft and the BPTB graft in microcalorimetry and colony counting. In the samples analyzed with electron microscopy, no specific biofilm growth pattern was identified upon comparing the BPTB graft to the 4×Ht graft. Conclusions: There were no significant differences found at either the quantitative or qualitative level when comparing bacterial growth in the BPTB graft to that in the 4×Ht graft. Therefore, the presence of sutures in the 4×Ht graft cannot be established as a predisposing factor for increased biofilm growth in this in vitro study.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Arthroscopy ; Sports medicine ; Anterior cruciate ligament reconstruction ; Implant-associated infection ; Biofilm ; Septic arthritis
Publicat a:	Pathogens, Vol. 12 (may 2023) , ISSN 2076-0817

DOI: 10.3390/pathogens12060761
PMID: 37375451

10 p, 2.2 MB

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