Enhanced Susceptibility to Tomato Chlorosis Virus (ToCV) in Hsp90- and Sgt1 -Silenced Plants : Insights from Gene Expression Dynamics
Ontiveros, Irene (Centre de Recerca en Agrigenòmica)
Fernández-Pozo, Noé (Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora")
Esteve-Codina, Anna (Centro Nacional de Análisis Genómico (Barcelona))
Lopez-Moya, Juan Jose (Centre de Recerca en Agrigenòmica)
Díaz Pendón, Juan Antonio (Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora")

Date:	2023
Abstract:	The emerging whitefly-transmitted crinivirus tomato chlorosis virus (ToCV) causes substantial economic losses by inducing yellow leaf disorder in tomato crops. This study explores potential resistance mechanisms by examining early-stage molecular responses to ToCV. A time-course transcriptome analysis compared naïve, mock, and ToCV-infected plants at 2, 7, and 14 days post-infection (dpi). Gene expression changes were most notable at 2 and 14 dpi, likely corresponding to whitefly feeding and viral infection. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed key genes and pathways associated with ToCV infection, including those related to plant immunity, flavonoid and steroid biosynthesis, photosynthesis, and hormone signaling. Additionally, virus-derived small interfering RNAs (vsRNAs) originating from ToCV predominantly came from RNA2 and were 22 nucleotides in length. Furthermore, two genes involved in plant immunity, Hsp90 (heat shock protein 90) and its co-chaperone Sgt1 (suppressor of the G2 allele of Skp1) were targeted through viral-induced gene silencing (VIGS), showing a potential contribution to basal resistance against viral infections since their reduction correlated with increased ToCV accumulation. This study provides insights into tomato plant responses to ToCV, with potential implications for developing effective disease control strategies.
Grants:	Agencia Estatal de Investigación PID2019-105692RB-I00 Agencia Estatal de Investigación PID2019-107657RB-C21 Agencia Estatal de Investigación PID2022-139376OB-C32 Agencia Estatal de Investigación PID2022-139376OB-C33 European Commission 101000570 Agencia Estatal de Investigación BES-2017-080808 Ministerio de Ciencia e Innovación RYC2020-030219-I Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Ciencia e Innovación CEX2019-000902-S
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Basal resistance ; Hsp90 ; Sgt1 ; ToCV ; Bemisia tabaci ; Tomato
Published in:	Viruses, Vol. 15, Issue 12 (December 2023) , art. 2370, ISSN 1999-4915

DOI: 10.3390/v15122370
PMID: 38140611

16 p, 2.3 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles