Development and Genome-Wide Analysis of a Blast-Resistant japonica Rice Variety
Escolà Oliva, Glòria (Centre de Recerca en Agrigenòmica)
González-Miguel, Víctor Manuel (Centre de Recerca en Agrigenòmica)
Campo, Sonia (Centre de Recerca en Agrigenòmica)
Catala-Forner, Mar (Institut de Recerca i Tecnologia Agroalimentàries)
Domingo, Concha (Instituto Valenciano de Investigaciones Agrarias)
Marqués, Luis (Cooperativa de Productores de Semillas de Arroz)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)

Data:	2023
Resum:	Rice is one of the most important crops in the world, and its production is severely affected by the rice blast disease caused by the fungus Magnaporthe oryzae. Several major blast resistance genes and QTLs associated with blast resistance have been described and mostly identified in indica rice varieties. In this work, we report the obtention of a blast-resistant rice breeding line derived from crosses between the resistant indica variety CT13432 and the japonica elite cultivar JSendra (highly susceptible to blast). The breeding line, named COPSEMAR9, was found to exhibit resistance to leaf blast and panicle blast, as demonstrated by disease assays under controlled and field conditions. Furthermore, a high-quality genome sequence of the blast-resistant breeding line was obtained using a strategy that combines short-read sequencing (Illumina sequencing) and long-read sequencing (Pacbio sequencing). The use of a whole-genome approach allowed the fine mapping of DNA regions of indica and japonica origin present in the COPSEMAR9 genome and the identification of parental gene regions potentially contributing to blast resistance in the breeding line. Rice blast resistance genes (including Pi33 derived from the resistant parent) and defense-related genes in the genome of COPSEMAR9 were identified. Whole-genome analyses also revealed the presence of microRNAs (miRNAs) with a known function in the rice response to M. oryzae infection in COPSEMAR9, which might also contribute to its phenotype of blast resistance. From this study, the genomic information and analysis methods provide valuable knowledge that will be useful in breeding programs for blast resistance in japonica rice cultivars.
Ajuts:	Agencia Estatal de Investigación RTI2018-101275-B-I00 Agencia Estatal de Investigación PID2021-128825OB-I00 Agencia Estatal de Investigación PID2019-104099RR-I00 Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Ciencia e Innovación CEX2019-000902-S Ministerio de Ciencia, Innovación y Universidades PEJ2018-002245-P
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Breeding ; Defense ; Genome mapping ; Japonica ; Magnaporthe oryzae ; Microrna (miRNA) ; Oryza sativa ; Resistance
Publicat a:	Plants, Vol. 12, Issue 20 (October 2023) art. 3536, ISSN 2223-7747

DOI: 10.3390/plants12203536
PMID: 37896000

19 p, 1.8 MB

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