Pro-inflammatory action of LDL(-) on mononuclear cells is counteracted by increased IL10 production
Benitez, Sonia (Institut d'Investigació Biomèdica Sant Pau)
Bancells, Cristina (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Ordóñez, J. (Jordi) 1952- (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Sanchez-Quesada, Jose Luis (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))

Data:	2007
Resum:	Objective: LDL(-) is a minor LDL subfraction that induces inflammatory factor release by endothelial cells. Since LDL(-) is present in plasma, its interaction with leucocytes, a cell type involved in atherosclerosis phenomena, is feasible; therefore, the aim of the current study was to evaluate LDL(-) effect on lymphocytes and monocytes isolated from human plasma. Methods and Results: Mononuclear cells were incubated with LDL(+) and LDL(-) and expression and release of several inflammatory mediators were analyzed by protein membrane assay, ELISA and real-time RT-PCR. LDL(-) induced a significantly increased production versus LDL(+) in MCP1, GROβ, GROγ, IL6, IL8 and IL10 in monocytes as well as in lymphocytes. These induced molecules are inflammatory, except for IL10 which is considered an anti-inflammatory cytokine. Therefore, the role of IL10 was evaluated in experiments where exogenous IL10 or antibodies anti-IL10 or anti-IL10 receptor were added. IL10 addition diminished the release of the other factors induced by LDL(-) near to basal production both at protein and RNA level. In contrast, the antibody anti-IL10 increased inflammatory cytokine release around two-fold, whereas the antibody anti-IL10 receptor produced a lower effect. Conclusions: LDL(-) promoted inflammatory cytokine production in leucocytes; however, it also induced IL10 that minimized this effect. Therefore, IL10 developed a significant role in counteracting the LDL(-) inflammatory action.
Ajuts:	Instituto de Salud Carlos III CP04/110 Instituto de Salud Carlos III PI03/0885
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Llengua:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Matèria:	LDL(-) ; Cytokine ; Monocyte ; Lymphocyte ; IL10
Publicat a:	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, Vol. 1771 Núm. 5 (may 2007) , p. 613-622, ISSN 1879-2618

DOI: 10.1016/j.bbalip.2007.03.001

22 p, 1.6 MB

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