Polylactic acid nanoplastics (PLA-NPLs) induce adverse effects on an in vitro model of the human lung epithelium : The Calu-3 air-liquid interface (ALI) barrier
García-Rodríguez, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Gutiérrez-García, Javier (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Villacorta, Aliro (Universidad Arturo Prat)
Arribas Arranz, Jéssica (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Romero i Andrada, Iris (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Lacoma, Alicia (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Rubio Lorente, Laura (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2024
Description:	16 pàg.
Abstract:	The expected increments in the production/use of bioplastics, as an alternative to petroleum-based plastics, require a deep understanding of their potential environmental and health hazards, mainly as nanoplastics (NPLs). Since one important exposure route to NPLs is through inhalation, this study aims to determine the fate and effects of true-to-life polylactic acid nanoplastics (PLA-NPLs), using the in vitro Calu-3 model of bronchial epithelium, under air-liquid interphase exposure conditions. To determine the harmful effects of PLA-NPLs in a more realistic scenario, both acute (24 h) and long-term (1 and 2 weeks) exposures were used. Flow cytometry results indicated that PLA-NPLs internalized easily in the barrier (∼10 % at 24 h and ∼40 % after 2 weeks), which affected the expression of tight-junctions formation (∼50 % less vs control) and the mucus secretion (∼50 % more vs control), both measured by immunostaining. Interestingly, significant genotoxic effects (DNA breaks) were detected by using the comet assay, with long-term effects being more marked than acute ones (7. 01 vs 4. 54 % of DNA damage). When an array of cellular proteins including cytokines, chemokines, and growth factors were used, a significant over-expression was mainly found in long-term exposures (∼20 proteins vs 5 proteins after acute exposure). Overall, these results described the potential hazards posed by PLA-NPLs, under relevant long-term exposure scenarios, highlighting the advantages of the model used to study bronchial epithelium tissue damage, and signaling endpoints related to inflammation.
Grants:	European Commission 801370 European Commission 965196 Agencia Estatal de Investigación PID2020-116789 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00731
Note:	Altres ajuts: acords transformatius de la UAB
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Nanoplastics ; Polylactic acid ; Callu-3 barrier ; Long-term exposure ; Air-liquid interphase
Published in:	Journal of hazardous materials, Vol. 475 (august 2024) , ISSN 1873-3336

DOI: 10.1016/j.jhazmat.2024.134900
PMID: 38878440

16 p, 10.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
Articles > Research articles
Articles > Published articles