Differences in enteric pathogens and intestinal microbiota between diarrheic weaned piglets and healthy penmates
Garcias Puigserver, Biel (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Migura-Garcia, Lourdes (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Giler Baquerizo, Noemí Esperanza (Universitat Autònoma de Barcelona. Unitat Mixta d'Investigació IRTA-UAB en Sanitat Animal)
Martín Castillo, Margarita (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Darwich Soliva, Laila (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)

Data:	2024
Resum:	Postweaning diarrhea (PWD) is a multifactorial disease caused by different aetiological agents, like viruses or bacteria and where the role of the microbiota remains unclear. The aim of this study was to assess differences between healthy and diarrheic weaned pigs concerning the prevalence of pathogens and changes in the intestinal microbiota. Eighteen farms with PWD were selected and 277 fecal samples were collected (152 diarrheic vs 125 healthy). Presence of Rotavirus A (RVA), B (RVB), C (RVC) and Porcine Epidemic Diarrhea Virus (PEDV), virulence factors of Escherichia coli and Clostridioides difficile were analyzed by PCR. Finally, the microbiota composition was also study by 16 S rRNA sequencing on 148 samples (102 diarrheic vs 46 healthy). RVA (53. 95 % vs 36 %, p=0. 04) and RVB (49. 67 % vs 28. 8 %, p<0. 001) were more frequent in diarrheic animals. Furthermore, RVA viral load was higher in diseased animals. VT2 toxin was significantly associated with diarrhea, whereas other virulence factors were not. Presence of C. difficile and PEDV was almost negligible. Regarding microbiota changes, Fusobacteriota phylum was more frequent in diarrheic samples and Ruminococcaceae family in healthy penmates. During the first week postweaning, Enterobacteriace and Campylobacteria were enriched in animals presenting diarrhea. Furthermore, Lactobacillus was detected in those individuals with no RVA infection. In conclusion, RVA seems to play a primary role in PWD. Classic E. coli virulence factors were not associated with diarrhea, indicating the need for revising their implication in disease. Moreover, Lactobacillus was found frequently in animals negative for RVA, suggesting some protective effect.
Ajuts:	Agencia Estatal de Investigación RTI2018-095586-B-C22 Agència de Gestió d'Ajuts Universitaris i de Recerca FI-SDUR2020
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Postweaning Diarrhea ; Escherichia coli ; Rotavirus ; Microbiota ; 16 S ; Swine ; Infectious diseases ; Lactobacillus
Publicat a:	Veterinary microbiology, Vol. 295 (august 2024) , p. 110162, ISSN 1873-2542

DOI: 10.1016/j.vetmic.2024.110162
PMID: 38941767

8 p, 2.5 MB

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