In Vitro Effect of Three-Antibiotic Combinations plus Potential Antibiofilm Agents against Biofilm-Producing Mycobacterium avium and Mycobacterium intracellulare Clinical Isolates
Batista, Sara (Hospital Clínic i Provincial de Barcelona)
Fernandez-Pittol, Mariana (Hospital Clínic i Provincial de Barcelona)
San Nicolás, Lorena (Hospital Clínic i Provincial de Barcelona)
Martínez, Diego (Hospital Clínic i Provincial de Barcelona)
Rubio, Marc (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Garrigo, Montserrat (Institut d'Investigació Biomèdica Sant Pau)
Vila Estapé, Jordi (Hospital Clínic i Provincial de Barcelona)
Tudó, Griselda (Hospital Clínic i Provincial de Barcelona)
Gonzalez-Martin, Julian (Hospital Clínic i Provincial de Barcelona)
Universitat Autònoma de Barcelona

Date:	2023
Abstract:	Patients with chronic pulmonary diseases infected by Mycobacterium avium complex (MAC) often develop complications and suffer from treatment failure due to biofilm formation. There is a lack of correlation between in vitro susceptibility tests and the treatment of clinical isolates producing biofilm. We performed susceptibility tests of 10 different three-drug combinations, including two recommended in the guidelines, in biofilm forms of eight MAC clinical isolates. Biofilm developed in the eight isolates following incubation of the inoculum for 3 weeks. Then, the biofilm was treated with three-drug combinations with and without the addition of potential antibiofilm agents (PAAs). Biofilm bactericidal concentrations (BBCs) were determined using the Vizion lector system. All selected drug combinations showed synergistic activity, reducing BBC values compared to those treated with single drugs, but BBC values remained high enough to treat patients. However, with the addition of PAAs, the BBCs steadily decreased, achieving similar values to the combinations in planktonic forms and showing synergistic activity in all the combinations and in both species. In conclusion, three-drug combinations with PAAs showed synergistic activity in biofilm forms of MAC isolates. Our results suggest the need for clinical studies introducing PAAs combined with antibiotics for the treatment of patients with pulmonary diseases infected by MAC.
Grants:	Ministerio de Economía y Competitividad PI16/01047 Instituto de Salud Carlos III PI22/00536
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Mycobacterium avium complex ; Antibiofilm agents ; Antibiotic combinations ; Antibiotics ; Biofilm
Published in:	Antibiotics, Vol. 12 Núm. 9 (september 2023) , p. 1409, ISSN 2079-6382

DOI: 10.3390/antibiotics12091409
PMID: 37760706

15 p, 2.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles