Low genetic diversity of Treponema pallidum ssp. pertenue (TPE) isolated from patients' ulcers in Namatanai District of Papua New Guinea : Local human population is infected by three TPE genotypes
Medappa, Monica (Masaryk University (República Txeca))
Pospíšilová, Petra (Department of Biology. Faculty of Medicine. Masaryk University)
Madruga, María Paula M. (Masaryk University (República Txeca))
John, Lucy N. (Universitat de Barcelona)
Beiras, Camila G. (Universitat de Barcelona)
Grillová, Linda (Masaryk University (República Txeca))
Oppelt, Jan (University of Pennsylvania)
Banerjee, Arka (Indian Institute of Technology)
Vall-Mayans, M. (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Mitjà, Oriol (University of Papua New Guinea)
Šmajs, David (Masaryk University (República Txeca))

Fecha:	2024
Resumen:	Yaws is an endemic disease caused by Treponema pallidum subsp. pertenue (TPE) that pri-marily affects children in rural regions of the tropics. The endemic character of yaws infections and the expected exclusive reservoir of TPE in humans opened a new opportunity to start a yaws eradication campaign. We have developed a multi-locus sequence typing (MLST) scheme for TPE isolates combining the previously published (TP0548, TP0488) and new (TP0858) chromosomal loci, and we compared this typing scheme to the two previously published MLST schemes. We applied this scheme to TPE-containing clinical isolates obtained during a mass drug administration study performed in the Namatanai District of Papua New Guinea between June 2018 and December 2019. Of 1081 samples collected, 302 (28. 5%) tested positive for TPE DNA, from which 255 (84. 4%) were fully typed. The TPE PCR-positivity in swab samples was higher in younger patients, patients with single ulcers, first ulcer episodes, and with ulcer duration less than six months. Non-treponemal serological test positivity correlated better with PCR positivity compared to treponema-spe-cific serological tests. The MLST revealed a low level of genetic diversity among infecting TPE isolates, represented by just three distinct genotypes (J11, S22, and T13). Two previously used typing schemes revealed similar typing resolutions. Two new alleles (one in TP0858 and one in TP0136) were shown to arise by intragenomic recombination/deletion events. Compared to samples genotyped as J11, the minor genotypes (T13 and S22) were more frequently detected in samples from patients with two or more ulcers and patients with higher values of specific TP serological tests. Moreover, the A2058G mutation in the 23S rRNA genes of three J11 isolates was found, resulting in azithromycin resistance.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Child ; Genotype ; Humans ; Multilocus Sequence Typing ; Mutation ; Papua New Guinea ; Treponema ; Treponema pallidum ; Treponema pallidum subsp. pertenue ; Ulcer ; Yaws
Publicado en:	PLoS neglected tropical diseases, Vol. 18 Núm. 1 (january 2024) , p. e0011831, ISSN 1935-2735

DOI: 10.1371/journal.pntd.0011831
PMID: 38166151

17 p, 1.7 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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