Impact of semen cryopreservation season on in vitro embryo production of prepubertal goat oocytes
Ferrer-Roda, Mònica (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Catalán, Jaime (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Izquierdo Tugas, Ma. Dolors (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Franco Oliveira, Maria Emilia (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Paramio, María Teresa (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)

Date:	2024
Abstract:	Goat production is affected by reproductive seasonality. In vitro embryo production (IVEP) could overcome this effect. This study aimed to evaluate the impact of the season of semen collection/freezing on IVEP of prepubertal goat oocytes and on sperm quality and functionality concerning capacitation. Semen from six fertile bucks was collected, pooled and cryopreserved in spring and autumn and used for IVEP of oocytes recovered during the breeding season. Oocytes were IVM in TCM-199 with hormones, EGF and cysteamine; fertilized and cultured in BO-IVF and BO-IVC media (IVF Bioscience, UK). Semen samples were assessed at 0 and 3 h after culture in capacitating (BO-IVF, CAP) and non-capacitating conditions for sperm plasma membrane and acrosome integrity, mitochondrial membrane potential (MMP), intracellular calcium and plasma membrane lipid disorder. Blastocyst production was higher with spring sperm compared to autumn (12. 0% vs. 2. 1%, respectively; p <. 05). After CAP, acrosome reaction and intracellular calcium were higher (p <. 05) in spring than autumn sperm. No differences were found in other sperm parameters. In conclusion, seasonal variations in the IVEP of prepubertal goats could be linked to differences in sperm ability to undergo in vitro capacitation.
Grants:	Agencia Estatal de Investigación PID2020-113266RB-I00
Note:	Altres ajuts: acords transformatius de la UAB ; European Union NextGenerationEU/PRTR (Juan de la Cierva Scheme: JDC2022-049684-I).
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Goat ; IVEP ; IVF ; Sperm capacitation
Published in:	Reproduction in domestic animals, Vol. 59 Núm. S3 (october 2024) , p. e14594, ISSN 1439-0531
Related work:	Ferrer Roda, Mònica; Paramio Nieto, Maria Teresa; Izquierdo Tugas, Dolors, 2024, "Replication Data for: "Impact of semen cryopreservation season on in vitro embryo production of prepubertal goat oocytes". CORA.Repositori de Dades de Recerca https://doi.org/10.34810/data1336

DOI: 10.1111/rda.14594
PMID: 39396865

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