| Resum: |
Objective: To determine the effect of marathon running on serum levels of inflammatory, high energy, and cartilage matrix biomarkers and to ascertain whether these biomarkers levels correlate. Design: Blood samples from 17 Caucasian male recreational athletes at the Barcelona Marathon 2017 were collected at the baseline, immediately and 48 h post-race. Serum C reactive protein (CRP), creatin kinase (CK), and lactate dehydrogenase (LDH) were determined using an AU-5800 chemistry analyser. Serum levels of hyaluronan (HA), cartilage oligomeric matrix protein (COMP), aggrecan chondroitin sulphate 846 (CS846), glycoprotein YKL-40, human procollagen II N-terminal propeptide (PIINP), human type IIA collagen N-propeptide (PIIANP), and collagen type II cleavage (C2C) were measured by sandwich enzyme-linked immune-sorbent assay (ELISA). Results: Medians CK and sLDH levels increased (three-fold, two-fold) post-race [429 (332) U/L, 323 (69) U/L] (p < 0. 0001; p < 0. 0001) and (six-fold, 1. 2-fold) 48 h post-race [658 (1,073) U/L, 218 (45) U/L] (p < 0. 0001; p < 0. 0001). Medians CRP increased (ten-fold) after 48 h post-race [6. 8 (4. 1) mg/L] (p < 0. 0001). Mean sHA levels increased (four-fold) post-race (89. 54 ± 53. 14 ng/ml) (p < 0. 0001). Means PIINP (9. 05 ± 2. 15 ng/ml) levels increased post-race (10. 82 ± 3. 44 ng/ml) (p = 0. 053) and 48 h post-race (11. 00 ± 2. 96 ng/ml) (p = 0. 001). Mean sC2C levels (220. 83 ± 39. 50 ng/ml) decreased post-race (188. 67 ± 38. 52 ng/ml) (p = 0. 002). In contrast, means COMP, sCS846, sPIIANP, and median sYKL-40 were relatively stable. We found a positive association between sCK levels with sLDH pre-race (r = 0. 758, p < 0. 0001), post-race (r = 0. 623, p = 0. 008) and 48-h post-race (r = 0. 842, p < 0. 0001); sHA with sCRP post-race vs. 48 h post-race (r = 0. 563, p = 0. 019) and sPIINP with sCK pre-race vs. 48-h post-race (r = 0. 499, p = 0. 044) and with sLDH 48-h pre-race vs. post-race (r = 0. 610, p = 0. 009) and a negative correlation of sPIIANP with sCRP 48-h post-race (r = -0. 570, p = 0. 017). Conclusion: Marathon running is an exercise with high-energy demands (sCK and sLDH increase) that provokes a high and durable general inflammatory reaction (sCRP increase) and an immediately post-marathon mechanism to protect inflammation and cartilage (sHA increase). Accompanied by an increase in type II collagen cartilage fibrils synthesis (sPIINP increase) and a decrease in its catabolism (sC2C decrease), without changes in non-collagenous cartilage metabolism (sCOMP, sC846, and sYKL-40). Metabolic changes on sPIINP and sHA synthesis may be related to energy consumption (sCK, sLDH) and the inflammatory reaction (sCRP) produced. |
visitant ::
identificació
(Universitat Autònoma de Barcelona. Departament de Cirurgia)
