Analysing polypeptide antibiotics residues in animal muscle tissues : The crucial role of HRMS
Rúbies, Antoni (Agència de Salut Pública de Barcelona)
Beguiristain, Iñaki (Universitat de Barcelona)
Tibon, Jojo (Universitat de Barcelona)
Cortés-Francisco, Nuria (Institut de Recerca Sant Pau)
Granados, Mercè (Universitat de Barcelona)

Date:	2024
Abstract:	A confirmatory method for the determination of polypeptide antibiotics (bacitracin, colistin, and polymyxin B) in muscle samples has been developed. Extraction is performed with acidified methanol, and a clean-up step by solid-phase extraction with polymeric cartridges is applied. Separation by ultra-high performance liquid chromatography (UHPLC) is carried out using a solid core C18 column and gradient elution with water/acetonitrile containing 0. 2% formic acid. High-resolution mass spectrometry (HRMS) (Q-Orbitrap) detection using different working modes has proved to be highly advantageous in eliminating interfering signals from endogenous matrix components. The analytical method has been successfully validated according to Commission Regulation 2021/808/EU and is currently used in a public health laboratory involved in veterinary medicines residue surveillance activities.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Bacitracin ; Colistin ; Food analysis ; Polymyxin B ; Q-Orbitrap
Published in:	Food chemistry, Vol. 443 (15 2024) , p. 138481, ISSN 1873-7072

DOI: 10.1016/j.foodchem.2024.138481
PMID: 38310677

9 p, 2.3 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Recerca Sant Pau
Articles > Research articles
Articles > Published articles