Hierarchical Surface Pattern on Ni-Free Ti-Based Bulk Metallic Glass to Control Cell Interactions
Cai, Fei-Fan (Montanuniversität Leoben)
Blanquer Jerez, Andreu (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Costa, Miguel B. (University of Cambridge)
Schweiger, Lukas (Montanuniversität Leoben)
Sarac, Baran (Austrian Academy of Sciences)
Greer, A. Lindsay (University of Cambridge)
Schroers, Jan (Yale University)
Teichert, Christian (Montanuniversität Leoben)
Nogués, Carme (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Spieckermann, Florian (Montanuniversität Leoben)
Eckert, Jürgen (Austrian Academy of Sciences)

Data:	2023
Resum:	Ni-free Ti-based bulk metallic glasses (BMGs) are exciting materials for biomedical applications because of their outstanding biocompatibility and advantageous mechanical properties. The glassy nature of BMGs allows them to be shaped and patterned via thermoplastic forming (TPF). This work demonstrates the versatility of the TPF technique to create micro- and nano-patterns and hierarchical structures on TiZrCuPdSn BMG. Particularly, a hierarchical structure fabricated by a two-step TPF process integrates 400 nm hexagonal close-packed protrusions on 2. 5 µm square protuberances while preserving the advantageous mechanical properties from the as-cast material state. The correlations between thermal history, structure, and mechanical properties are explored. Regarding biocompatibility, TiZrCuPdSn BMGs with four surface topographies (flat, micro-patterned, nano-patterned, and hierarchical-structured surfaces) are investigated using Saos-2 cell lines. Alamar Blue assay and live/dead analysis show that all tested surfaces have good cell proliferation and viability. Patterned surfaces are observed to promote the formation of longer filopodia on the edge of the cytoskeleton, leading to star-shaped and dendritic cell morphologies compared with the flat surface. In addition to potential implant applications, TPF-patterned Ti-BMGs enable a high level of order and design flexibility on the surface topography, expanding the available toolbox for studying cell behavior on rigid and ordered surfaces. A hierarchical structure integrating nano-scale protrusions on micro-scale protuberances is achieved on Ni-free Ti-based bulk metallic glasses via a two-step thermoplastic forming technique. The patterned materials preserve advantageous mechanical properties and biocompatibility from the as-cast materials. However, the surface features change the cell morphology. Besides implant applications, this work realizes a strategy for studying cell behavior on rigid ordered surfaces.
Ajuts:	Agencia Estatal de Investigación PID2020-116844RB-C21 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00122 European Commission 861046
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Biocompatibility ; Biomaterials ; Bulk metallic glass ; Patterning ; Thermoplastic forming ; Titanium alloys ; Topography
Publicat a:	Small (Weinheim), Vol. 20 (december 2023) , ISSN 1613-6829

DOI: 10.1002/smll.202310364
PMID: 38109153

13 p, 4.4 MB

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