Nucleic acid degradation after long-term dried blood spot storage
Li, Juan (Leibniz Institute for Zoo and Wildlife Research)
Ulloa, Gabriela M. (Universidade Federal Rural da Amazônia)
Mayor Aparicio, Pedro Ginés (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Santolalla Robles, Meddly L. (Universidad Peruana Cayetano Heredia)
Greenwood, Alex D. (Freie Universität Berlin)

Data:	2024
Resum:	Collecting and preserving biological samples in the field, particularly in remote areas in tropical forests, prior to laboratory analysis is challenging. Blood samples in many cases are used for nucleic acid-based species determination, genomics or pathogen research. In most cases, maintaining a cold chain is impossible and samples remain at ambient temperature for extended periods of time before controlled storage conditions become available. Dried blood spot (DBS) storage, blood stored on cellulose-based paper, has been widely applied to facilitate sample collection and preservation in the field for decades. However, it is unclear how long-term storage on this substrate affects nucleic acid concentration and integrity. We analysed nucleic acid quality from DBS stored on Whatman filter paper no. 3 and FTA cards for up to 15 years in comparison to cold-chain stored samples using four nucleic acid extraction methods. We examined the ability to identify viral sequences from samples of 12 free-ranging primates in the Amazon forest, using targeted hybridization capture, and determined if mitochondrial genomes could be retrieved. The results suggest that even after extended periods of storage, DBS will be suitable for some genomic applications but may be of limited use for viral pathogen research, particularly RNA viruses.
Nota:	Altres ajuts:DLR ERANet- LAC [grant 17/HLH- 0271] within the research project (Contract N° 136- 2018- FONDECYT; AC18/00054 Instituto de Salud Carlos; CNPq - 400800/2019- 5). Juan Li: China Scholarship Council. Gabriela M. Ulloa: fellowships from the program PPGSPAA by the CNPq in GD modality (140312/2020- 0) and SWE modality (201546/2020- 5). Open Access funding enabled and organized by Projekt DEAL.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	DBS ; Hybridization capture ; Mitochondrial genome ; Nucleic acid ; Virus
Publicat a:	Molecular ecology resources, Vol. 24 Núm. 6 (2024) , p. e13979, ISSN 1755-0998

DOI: 10.1111/1755-0998.13979
PMID: 38780145

14 p, 1.5 MB

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