Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size
Ferrer-Roda, Mònica (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Gil, Ana (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Paramio, María Teresa (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Izquierdo Tugas, Ma. Dolors (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments

Date:	2024
Description:	11 pàg.
Abstract:	Oocytes spontaneously resume meiosis following their liberation from follicles, preventing full competence acquisition. Biphasic IVM (CAPA-IVM) maintains oocytes in meiotic arrest to improve developmental competence, and it specially affects poorly developed oocytes. We assessed the effect of CAPA-IVM on oocytes from small (<3mm) and large (>3mm) follicles of prepubertal goats. Oocytes were cultured for 6h in pre-IVM with C-type natriuretic peptide (CNP) and estradiol as meiotic inhibitors, and germinal vesicle (GV) rate and chromatin configuration were assessed. Then, oocytes were cultured in conventional IVM (c-IVM) or CAPA-IVM (pre-IVM + c-IVM) and EGF receptor (EGFR) protein expression, intra-oocyte ROS and blastocyst development were assessed. GV rate was higher in CNP groups than control (69% vs 28%, and 67% vs 31%, small and large follicles, respectively), but GV chromatin configuration was similar. In large follicles, EGFR expression was higher in oocytes and cumulus cells after CAPA-IVM, and ROS levels were lower. In small follicles these differences were not observed. c-IVM and CAPA-IVM produced similar blastocyst rates in small (3. 7% vs 2. 6%, respectively) and large follicles (8. 3% vs 2. 5%). Overall, CAPA-IVM enhanced EGFR expression for EGF peptide signalling and antioxidant capacity in oocytes from large follicles but oocytes from small follicles were too immature to benefit from it.
Grants:	Agencia Estatal de Investigación PID2020-113266RB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca FI-B00025
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	GV chromatin configuration ; Oocyte competence ; Follicle diameter ; EGFR ; Epidermal growth factor ; Blastocyst production
Published in:	Animal Biotechnology, Vol. 35 Núm. 1 (2024) , p. 2422316, ISSN 1532-2378
Related work:	Ferrer Roda, Mònica; Paramio Nieto, Maria Teresa; Izquierdo Tugas, Dolors, 2024, "Replication Data for: Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size". CORA.Repositori de Dades de Recerca https://doi.org/10.34810/data1389

DOI: 10.1080/10495398.2024.2422316
PMID: 39522054

12 p, 1.6 MB

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