Non-canonical LexA proteins regulate the SOS response in the Bacteroidetes
Sánchez-Osuna, Miquel (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Cortés Garmendia, M. Pilar (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Lee, Mark (University of Maryland Baltimore County. Department of Chemistry and Biochemistry)
Smith, Aaron T. (University of Maryland Baltimore County. Department of Chemistry and Biochemistry)
Barbé García, Jordi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Erill, Ivan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2021
Resum:	Lesions to DNA compromise chromosome integrity, posing a direct threat to cell survival. The bacterial SOS response is a widespread transcriptional regulatory mechanism to address DNA damage. This response is coordinated by the LexA transcriptional repressor, which controls genes involved in DNA repair, mutagenesis and cell-cycle control. To date, the SOS response has been characterized in most major bacterial groups, with the notable exception of the Bacteroidetes. No LexA homologs had been identified in this large, diverse and ecologically important phylum, suggesting that it lacked an inducible mechanism to address DNA damage. Here, we report the identification of a novel family of transcriptional repressors in the Bacteroidetes that orchestrate a canonical response to DNA damage in this phylum. These proteins belong to the S24 peptidase family, but are structurally different from LexA. Their N-terminal domain is most closely related to CI-type bacteriophage repressors, suggesting that they may have originated from phage lytic phase repressors. Given their role as SOS regulators, however, we propose to designate them as non-canonical LexA proteins. The identification of a new class of repressors orchestrating the SOS response illuminates long-standing questions regarding the origin and plasticity of this transcriptional network.
Ajuts:	Agencia Estatal de Investigación BIO2016-77011-R
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Publicat a:	Nucleic Acids Research, Vol. 49, Issue 19 (November 2021) , p. 11050-11066, ISSN 0305-1048

DOI: 10.1093/nar/gkab773
PMID: 34614190

17 p, 7.0 MB

El registre apareix a les col·leccions:
Articles > Articles de recerca
Articles > Articles publicats