Lymphaticovenous anastomosis in rabbits : A novel live experimental animal model for supermicrosurgical training
Campos, José L. (Centro de Cirugía de Mínima Invasión Jesús Usón)
Pires, Laura (Centro de Cirugía de Mínima Invasión Jesús Usón)
Vela, Francisco J. (Centro de Cirugía de Mínima Invasión Jesús Usón)
Pons, Gemma (Institut de Recerca Sant Pau)
Al-Sakkaf, Ali Mahdi Al-Haddar (Hospital de la Santa Creu i Sant Pau (Barcelona, Catalunya))
Sánchez-Margallo, Francisco M. (Centro de Cirugía de Mínima Invasión Jesús Usón)
Abellán, Elena (Centro de Cirugía de Mínima Invasión Jesús Usón)
Masià, Jaume (Institut de Recerca Sant Pau)
Universitat Autònoma de Barcelona

Fecha:	2024
Resumen:	Lymphaticovenous anastomosis is widely used in lymphedema management. Although its effectiveness in reducing edema in patients can be clinically observed, evaluating the long-term outcomes of this technique can be complex. This study established an animal model to assess the outcomes of lymphaticovenous anastomosis technique at 15 and 30-days post-surgery using indocyanine green lymphography, Patent Blue V dye injection, and histopathological examination. An experimental model was established in the hindlimbs of 10 rabbits using the popliteal vein and afferent lymphatic vessels in the popliteal area. The subjects were divided into two groups: the first group (n = 5) underwent patency assessment at 0 and 15 days, and the second group (n = 5) at 0 and 30-days, resulting in 20 anastomoses. Patency was verified at 0, 15, and 30-days using indocyanine green lymphography and Patent Blue V injection. Histopathological examinations were performed on the collected anastomosis samples. The patency rate was 90% (19/20) initially, 60% (6/10) at 15 days post-surgery, and 80% (8/10) at 30-days. The average diameter of lymphatic vessels and veins was 1. 0 mm and 0. 8 mm, respectively. The median number of collateral veins was 3; the median surgical time was 65. 8 min. Histopathology revealed minimal endothelial damage and inflammatory responses due to the surgical sutures, with vascular inflammation and thrombosis in a single case. Local vascular neoformations were observed. This study highlights the reliability and reproducibility of using rabbits as experimental models for training in lymphaticovenous anastomosis technique owing to the accessibility of the surgical site and dimensions of their popliteal vasculature.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Lymphaticovenous anastomosis ; Lymphedema ; Rabbit animal model ; Supermicrosurgery
Publicado en:	Journal of Plastic, Reconstructive and Aesthetic Surgery, Vol. 93 (june 2024) , p. 290-298, ISSN 1878-0539

DOI: 10.1016/j.bjps.2024.04.023
PMID: 38754281

9 p, 8.5 MB

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