Comparative analysis of anticoagulant influence on PMI estimation based on porcine blood metabolomics profile measured using GC-MS
Mojsak, Patrycja (Medical University of Bialystok (Polònia))
Samczuk, Paulina (Medical University of Bialystok (Polònia))
Klimaszewska, Paulina (Medical University of Bialystok (Polònia))
Burdukiewicz, Michał (Universitat Autònoma de Barcelona)
Chilimoniuk, Jaroslaw (Medical University of Bialystok (Polònia))
Grzesiak, Krystyna (University of Wroclaw (Polònia))
Pietrowska, Karolina (Medical University of Bialystok (Polònia))
Ciborowska, Justyna (Forensic Laboratory of the Voivodeship Police Headquarters in Bialystok (Polònia))
Niemcunowicz-Janica, Anna (Medical University of Bialystok (Polònia))
Kretowski, Adam (Medical University of Bialystok (Polònia))
Ciborowski, Michal (Medical University of Bialystok (Polònia))
Szeremeta, Michal (Medical University of Bialystok (Polònia))

Data:	2025
Resum:	Accurate post-mortem interval (PMI) estimation is essential in forensic investigations. Although various methods for PMI determination have been developed, only an approximate estimation is still achievable, and an accurate PMI indication is still challenging. Therefore, in this study, we employed gas chromatography-mass spectrometry (GC-MS)-based metabolomics to assess post-mortem changes in porcine blood samples collected with and without the addition of anticoagulant (EDTA). Our study aimed to identify metabolites dependent on the EDTA addition and time (taking into account the biodiversity of the studied organism) and those that are time-dependent but resistant to the addition of an anticoagulant. The experiment was performed on blood samples collected from 16 animals (domestic pig, breed: Polish Large White), 8 with and 8 without EDTA addition. The moment of death (time 0) and 15 additional time points (from 3 to 168 h after death) were selected to examine changes in metabolites' levels in specific time intervals. We employed linear mixed models to study the relationship between metabolite intensities, time and presence of EDTA while accounting for the effect of individual pigs. We confirmed that the intensity of 16 metabolites (mainly amino acids) significantly depends on PMI and the presence of EDTA. However, the intensity of the ideal biomarker(s) for PMI estimation should be determined only by the time after death and not by external factors such as the presence of the anticoagulant agent. Thus, we identified 41 metabolites with time-dependent intensities that were not susceptible to EDTA presence. Finally, we assessed the performance of these metabolites in a PMI predictive model. Citraconic acid yielded one of the lowest errors in general PMI estimation (32. 82 h). Moreover, similar errors were observed for samples with and without EDTA (33. 32 h and 32. 34 h, respectively). Although the small sample size and information leak in predictive modelling prevent drawing definite conclusions, citraconic acid shows potential as a robust PMI estimator.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Post-mortem interval (PMI) ; Animal model ; Blood biomarkers ; Metabolomics ; GC-MS
Publicat a:	Frontiers in Molecular Biosciences, Vol. 11 (january 2025) , ISSN 2296-889X

DOI: 10.3389/fmolb.2024.1400622
PMID: 39840077

10 p, 24.8 MB

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