Clot signature in patients with large vessel occlusion stroke and concomitant active cancer
Woock, Malin (Sahlgrenska University Hospital)
Rossi, Rosanna (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Jabrah, Duaa (University of Galway. Department of Physiology)
Douglas, Andrew (University of Galway. Department of Physiology)
Redfors, Petra (Sahlgrenska University Hospital)
Nordanstig, Annika (Sahlgrenska University Hospital)
Tatlisumak, Turgut (Sahlgrenska University Hospital)
Ceder, Erik (Sahlgrenska University Hospital. Department of Radiology)
Dunker, Dennis (Sahlgrenska University Hospital. Department of Radiology)
Carlqvist, Jeanette (Sahlgrenska University Hospital. Department of Radiology)
Szikora, István (National Institute of Clinical Neurosciences (Budapest). Department of Neurointerventions)
Tsivgoulis, Georgios (University General Hospital Attikon (Haidari, Grècia))
Psychogios, Klearchos (Metropolitan Hospital (Piraeu). Stroke Unit)
Magoufis, Georgios (Metropolitan Hospital (Piraeu). Stroke Unit)
Rentzos, Alexandros (Sahlgrenska University Hospital. Department of Radiology)
Doyle, Karen M. (University of Galway. Department of Physiology)
Jood, Katarina (Sahlgrenska University Hospital)

Date:	2025
Abstract:	Patients with active cancer face an increased risk of ischemic stroke. Also, stroke may be an initial indicator of cancer. In patients with large vessel occlusion (LVO) stroke treated with thrombectomy, analysis of the clot composition may contribute new insights into the pathological connections between these two conditions. We compared the content of 64 consecutively retrieved clots from LVO stroke patients with concomitant active cancer and 64 clots from matched-control LVO stroke patients without a history of cancer. Clots were analyzed with respect to histological composition by Martius Scarlet Blue, von Willebrand factor (vWF), citrullinated histone H3 (H3Cit, a biomarker of NETS), CD42b, and CD3 expression by immunohistochemistry. Orbit Image Analysis was used for quantification. Differences between groups were tested using the Mann-Whitney U -test and Chi-square Test. Clots from patients with concomitant cancer had a significantly higher content of vWF (median 26 [IQR13-38]% vs. 10 [4-18]%, p < 0. 0001) and H3Cit (median 0. 11 [IQR0. 02-0. 46]% vs. 0. 05 [0. 00-0. 28]% p = 0. 027) than controls. The presence of collagen >1% within the retrieved clots was highly indicative of cancer, occurring in 16/64 with active cancer and in 3/64 controls, p = 0. 002. After correction for multiple comparisons, the statistical significance for H3Cit was lost. Red and white blood cells, platelets, fibrin, and expression of CD3 and CD42b did not differ between the groups. Clots from LVO patients with concomitant active cancer possess distinct characteristics, indicating an influence of cancer on the innate immune system, fibroblasts, and the vascular endothelium in the formation of LVO clots.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Acute ischemic stroke & cancer ; Clot composition ; Collagen ; NETS ; Vwf
Published in:	European Journal of Neurology, Vol. 32, issue 1 (January 2025) , art. e70037, ISSN 1468-1331

DOI: 10.1111/ene.70037
PMID: 39760182

10 p, 822.3 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
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