Natural outbreak of Mycobacterium caprae infection in imported laboratory cynomolgus macaques (Macaca fascicularis) : diagnostic pitfalls and management of safety precautions
Weber, Klaus (AnaPath Services Gmbh)
Mayoral, Francisco José (AnaPath Research S.A.U.)
Vallejo, Carla (AnaPath Research S.A.U.)
Sánchez, Raúl (AnaPath Research S.A.U.)
Hartelust, Roberto (Hartelust & Co.)
Mendoza, Paula (AnaPath Research S.A.U.)
Pérez de Val, Bernat (Unitat mixta d'investigació IRTA-UAB en Sanitat Animal. Centre de Recerca en Sanitat Animal)
Savé, Jordi (AnaPath Research S.A.U.)
Okazaki, Yoshimasa (AnaPath Services GmbH)
Ortega, Paula (AnaPath Services GmbH)
Rocamora, Laura (AnaPath Research S.A.U.)
Sandoval, Albert (AnaPath Research S.A.U.)
Vallejo, Raquel (AnaPath Services GmbH)
de Miguel, Ricardo (AnaPath Services GmbH)
Kegler, Kristel (AnaPath Services GmbH)

Date:	2024
Abstract:	Tuberculosis (TB) is a major health threat for humans and for non-human primates used for toxicology or research purposes. Emerging mycobacterial species represent a major challenge for diagnosis and surveillance programs. Here, we report a natural outbreak of Mycobacterium caprae in imported cynomolgus macaques (Macaca fascicularis) that occurred at AnaPath Research S. A. U. (APR). The macaques underwent repeated negative intradermal tuberculin tests (IDT) before importation and at the European quarantine station. Exhaustive TB screening was started at APR after confirmation of one positive case at another facility. The animal in question belonged to the same colony received at APR. Diagnostic approaches included clinical examination, PCR, culture, spoligotyping, IDT testing, interferon-γ release assay (IGRA), and thoracoabdominal ultrasound (US). Three regulatory toxicity studies and stock animals were affected. The macaques lacked clinical signs, except for one showing a fistulizing nodule in the right inguinal area, which tested positive for the Mycobacterium tuberculosis complex by PCR. All animals were necropsied and 10 macaques (n=114) showed gross and histologic findings compatible with TB confirmed by PCR and culture. M. caprae was identified as the etiological agent by Direct Variable Repeat spacer oligonucleotide typing (DVR spoligotyping). The infection was traced to Asia via the SB1622 spoligotype involved, confirming that the animals were infected prior to their import into Europe. Tuberculin skin test (TST), IGRA, and US were only sensitive in detecting advanced cases of M. caprae infection. One staff member showed a positive TST reaction, which was handled in accordance with the Spanish government's health regulations. All the sanitary measures implemented were effective in eradicating the disease.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Tuberculosis ; Cynomolgus monkey ; Diagnostics ; Spoligotyping ; Prophylactic measures
Published in:	Journal of Toxicologic Pathology, Vol. 37 (july 2024) , p. 197-206, ISSN 1881-915X

DOI: 10.1293/tox.2024-0048
PMID: 39359895

10 p, 4.7 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Research articles
Articles > Published articles