Solid-state fermentation to produce biostimulant agents from green waste : A circular approach at bench-scale
Ghoreishi, Golafarin (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Barrena Gómez, Raquel (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sanchez, Antoni (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Font i Segura, Xavier (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Data:	2024
Resum:	This study aimed to develop a bench-scale process of solid-state fermentation (SSF) to produce biostimulants (indole-3-acetic acid-IAA) by Trichoderma harzianum CECT 2929, utilizing green waste as substrate. Additionally, biopesticide activity (in form of conidial spores) was also studied. The SSF was carried out in a 22-L packed bed reactor using grass clippings as the main substrate and pruning waste or wood chips as bulking agent. When using pruning waste, IAA production reached 62 µg/g dry matter, while the fermentation with wood chips resulted in a higher IAA concentration of 120 µg/g dry matter. Higher spore counts were reached using wood chips (9. 5 × 10 and 1. 3 × 10 spores g dry matter, respectively). Further experiments showed that decreasing tryptophan as precursor for IAA production from 0. 43 % to 0. 2 % (w/w) significantly decreased IAA production from 120 to 25 µg/g dry matter while it did not alter spore production. In addition, a sequential batch operational strategy was explored, consisting of four consecutive batches as a strategy to scale-up the SSF process. The highest IAA and spore productions were achieved in the first batch, resulting in 119 µg/g dry matter and 1. 1 × 10 spores g dry matter, respectively. Subsequent batches showed a decline in production, particularly in batches 3 and 4, due to contamination issues. Summarizing, the strategies proposed in this study are a considerable advance to develop SSF to produce biostimulant and biopesticide agents in the context of circular bioeconomy, using green waste as raw material.
Ajuts:	Agencia Estatal de Investigación PLEC2022-009252
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Indole-3-acetic acid ; Biostimulant ; Packed bed reactor ; Sequential batch reactor ; Trichoderma harzianum ; Biopesticide
Publicat a:	Chemical engineering journal (1996), Vol. 502 (December 2024) , art. 158057, ISSN 1873-3212

DOI: 10.1016/j.cej.2024.158057

11 p, 4.6 MB

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