Teabag-derived micro/nanoplastics (true-to-life MNPLs) as a surrogate for real-life exposure scenarios
Banaei, Gooya (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Abass, Doaa (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Tavakolpournegari, Alireza (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Martín Pérez, Joan (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Gutiérrez-García, Javier (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Peng, Guyu (Helmholtz Centre for Environmental Research)
Reemtsma, Thorsten (Helmholtz Centre for Environmental Research)
Marcos Dauder, Ricardo (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Hernández Bonilla, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
García Rodríguez, Alba (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Data:	2024
Resum:	The potential health implications of environmental micro/nanoplastics (MNPLs) are increasingly concerning. Beyond environmental exposure, other sources such as food packaging, including herbal/teabags, may also be significant. This study investigates the release of MNPLs from three commercially available teabags. By simulating tea preparation, MNPL samples were extracted and characterized using a range of analytical techniques: scanning electron microscopy (SEM), transmission electron microscopy (TEM), attenuated total reflectance/Fourier transform infrared spectroscopy (ATR-FTIR), dynamic light scattering (DLS), laser Doppler velocimetry (LDV), and nanoparticle tracking analysis (NTA). The results confirmed that the teabags were made of nylon-6 (NY6), polypropylene (PP), and cellulose (CL) and that microfibers and nano-range particles (NPLs) were present in the leachates. NTA data revealed that the number of released NPLs was 1. 20 × 109/mL (PP; 136. 7 nm), 1. 35 × 108/mL (CL; 244 nm), and 8. 18 × 106/mL (NY6; 138. 4). The leachate particles were then stained with iDye Poly-Pink and used to expose three human intestine-derived cell types (Caco-2, HT29, and HT29-MTX) to assess their biointeractions and the role of the mucosubstances in vitro. The results demonstrated that after 24 h of exposure to 100 μg/mL NPLs, there was significant uptake of PP-NPLs in HT29-MTX cells, as a model of cells segregating high amount of mucus. A similar uptake was observed for CL-NPLs in HT29 and HT29-MTX cells, while NY6-NPLs were internalized preferentially in Caco-2 cells. These findings underscore the importance of identifying new environmentally relevant MNPL exposure sources, for developing realistic MNPLs samples, and further investigating their potential human health effects.
Ajuts:	European Commission 801370 European Commission 965196 Agencia Estatal de Investigación PID2020-116789RB-C43 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-00731
Nota:	Altres ajuts: acords transformatius de la UAB
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Cell uptake ; Cellulose ; Intestinal cells ; Nanoplastics ; Nylon ; Polypropylene ; Teabags ; SDG 3 - Good Health and Well-being
Publicat a:	Chemosphere, Vol. 368 (November 2024) , art. 143736, ISSN 1879-1298

DOI: 10.1016/j.chemosphere.2024.143736
PMID: 39542373

12 p, 11.1 MB

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