Arrhythmic Effects Evaluated on Caenorhabditis elegans : The Case of Polypyrrole Nanoparticles
Srinivasan, Sumithra Yasaswini (Universitat Autònoma de Barcelona)
Illera, Pilar Alvarez (Universidad de Valladolid)
Kukhtar, Dmytro (Institut d'Investigació Biomèdica de Bellvitge)
Benseny Cases, Núria (Laboratori de Llum del Sincrotró ALBA)
Cerón, Julián (Institut d'Investigació Biomèdica de Bellvitge)
Álvarez, Javier (Universidad de Valladolid)
Fonteriz, Rosalba I. (Universidad de Valladolid)
Montero, Mayte (Universidad de Valladolid)
Laromaine, Anna (Universitat Autònoma de Barcelona)

Data:	2023
Resum:	Experimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organisms such as the Caenorhabditis elegans (C. elegans) can bridge the gap between in vitro and vertebrate testing as they can provide extensive information on systemic toxicity and specific harmful effects through facile experimentation following 3R EU directives on animal use. Since the nematodes' pharynx shares similarities with the human heart, we assessed the general and pharyngeal effects of drugs and polypyrrole nanoparticles (Ppy NPs) using C. elegans. The evaluation of FDA-approved drugs, such as Propranolol and Racepinephrine reproduced the arrhythmic behavior reported in humans and supported the use of this small animal model. Consequently, Ppy NPs were evaluated due to their research interest in cardiac arrhythmia treatments. The NPs' biocompatibility was confirmed by assessing survival, growth and development, reproduction, and transgenerational toxicity in C. elegans. Interestingly, the NPs increased the pharyngeal pumping rate of C. elegans in two slow-pumping mutant strains, JD21 and DA464. Moreover, the NPs increased the pumping rate over time, which sustained up to a day post-excretion. By measuring pharyngeal calcium levels, we found that the impact of Ppy NPs on the pumping rate could be mediated through calcium signaling. Thus, evaluating arrhythmic effects in C. elegans offers a simple system to test drugs and nanoparticles, as elucidated through Ppy NPs.
Ajuts:	European Commission 754397 European Commission 101057527 Agencia Estatal de Investigación PID2021-122239OB-I00
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Polypyrrole nanoparticles ; C. Elegans ; Small animal models ; Arrhythmia models ; Cardiotoxicity
Publicat a:	ACS nano, Vol. 17, Issue 17 (August 2023) , p. 17273-17284, ISSN 1936-086X

DOI: 10.1021/acsnano.3c05245
PMID: 37624669

12 p, 7.4 MB

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