Exon-intron split analysis reveals posttranscriptional regulatory signals induced by high and low n-6/n-3 polyunsaturated fatty acid ratio diets in piglets
Manaig, Yron Joseph (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Mármol-Sánchez, Emilio (Stockholm University. Department of Molecular Biosciences)
Castelló Farré, Anna (Centre de Recerca en Agrigenòmica)
Esteve-Codina, Anna (Institut de Ciència i Tecnologia de Barcelona)
Sandrini, Silvia (Università degli Studi di Milano. Department of Veterinary Medicine and Animal Sciences)
Savoini, Giovanni (Università degli Studi di Milano. Department of Veterinary Medicine and Animal Sciences)
Agazzi, Alessandro (Università degli Studi di Milano. Department of Veterinary Medicine and Animal Sciences)
Sánchez Bonastre, Armando (Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments)
Folch, Josep M (Centre de Recerca en Agrigenòmica)

Date:	2023
Abstract:	Polyunsaturated fatty acids (PUFA), such as omega-6 (n-6) and omega-3 (n-3), play a vital role in nutrient metabolism, inflammatory response, and gene regulation. microRNAs (miRNA), which can potentially degrade targeted messenger RNAs (mRNA) and/or inhibit their translation, might play a relevant role in PUFA-related changes in gene expression. Although differential expression analyses can provide a comprehensive picture of gene expression variation, they are unable to disentangle when in the mRNA life cycle the regulation of expression is taking place, including any putative functional miRNA-driven repression. To capture this, we used an exon-intron split analysis (EISA) approach to account for posttranscriptional changes in response to extreme values of n-6/n-3 PUFA ratio. Longissimus dorsi muscle samples of male and female piglets from sows fed with n-6/n-3 PUFA ratio of 13:1 (SOY) or 4:1 (LIN), were analyzed in a bidirectional contrast (LIN vs. SOY, SOY vs. LIN). Our results allowed the identification of genes showing strong posttranscriptional downregulation signals putatively targeted by significantly upregulated miRNA. Moreover, we identified genes primarily involved in the regulation of lipid-related metabolism and immune response, which may be associated with the pro- and anti-inflammatory functions of the n-6 and n-3 PUFA, respectively. EISA allowed us to uncover regulatory networks complementing canonical differential expression analyses, thus providing a more comprehensive view of muscle metabolic changes in response to PUFA concentration.
Grants:	European Commission 765423 Agencia Estatal de Investigación AGL2017-82641-R Agencia Estatal de Investigación PID2020-112677RB-C22 Agencia Estatal de Investigación CEX2019-000902-S
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Exon-intron split analysis ; MicroRNA ; Messenger RNA ; Piglets ; PUFA
Published in:	Journal of animal science, Vol. 101 (August 2023) , art. skad271, ISSN 1525-3163

DOI: 10.1093/jas/skad271
PMID: 37561402

12 p, 628.9 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles