Ganoderma lucidum Immobilized on Wood Demonstrates High Persistence During the Removal of OPFRs in a Trickle-Bed Bioreactor
Tayar, Shamim (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Villagra, Javier (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Gaju, Núria (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Martínez Alonso, María Ramos (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Beltrán-Flores, Eduardo (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Sarrà i Adroguer, Montserrat (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Data:	2025
Resum:	Emerging pollutants such as organophosphate flame retardants (OPFRs) pose a critical threat to environmental and human health, while conventional wastewater treatments often fail to remove them. This study addresses this issue by evaluating the bioremediation potential of white-rot fungi for the removal of two OPFRs: tris(2-chloroethyl) phosphate (TCEP) and tributyl phosphate (TBP). Three fungal species- Ganoderma lucidum, Trametes versicolor, and Phanerochaete velutina -were screened for their degradation capabilities. Among these, G. lucidum and T. versicolor demonstrated removal efficiencies exceeding 99% for TBP, while removal rates for TCEP were significantly lower, with a maximum of 30%. The exploration of the enzyme role showed that cytochrome P450 is involved in the degradation while the extracellular laccase is not involved. Continuous batch experiments were performed using a trickle-bed reactor (TBR) operating under non-sterile conditions, a setting that closely resembles real-world wastewater treatment environments. G. lucidum was immobilized on oak wood chips, and the removal efficiencies were measured to be 85. 3% and 54. 8% for TBP and TCEP, respectively, over 10 cycles. Microbial community analysis showed that G. lucidum remained the dominant species in the reactor. These findings demonstrate the efficacy of fungal-based trickle-bed bioreactors, offering a sustainable and efficient alternative for addressing environmental pollution caused by highly recalcitrant pollutants.
Ajuts:	Agencia Estatal de Investigación PID2022-138929OB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca 2021/SGR-01008
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Flame retardants ; Bioreactor ; Immobilization ; White-rot fungi ; Microbial assessment
Publicat a:	Journal of Fungi, Vol. 11, Issue 2 (January 2025) , art. 85, ISSN 2309-608X

DOI: 10.3390/jof11020085
PMID: 39997379

21 p, 1.7 MB

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