Comparison of Methods for Isolating Exosomes from Plasma Subjects with Normal and High Fat Percentages †
Noboa-Velástegui, Jacqueline Alejandra (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
León Contreras, Juan Carlos (Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (Mèxic))
Castro, Jorge (Centro Universitario de la Costa Sur (Mèxic))
Fletes Rayas, Ana Lilia (Universidad de Guadalajara (Mèxic))
Madrigal, Perla (Universidad de Guadalajara (Mèxic))
Álvarez, Iñaki (Universitat Autònoma de Barcelona. Institut de Biotecnologia i de Biomedicina "Vicent Villar Palasí")
Navarro Hernández, Rosa Elena (Universidad de Guadalajara (Mèxic))

Date:	2025
Abstract:	Adipose tissue is responsible for fat storage and is an important producer of extracellular vesicles (EVs). The biological content of exosomes, one kind of EV, provides information on aspects such as immunometabolic alterations. This study aimed to compare three plasma exosome isolation methods-using a commercial kit (CK), size exclusion chromatography (SEC), and differential centrifugation (DC)-and select the best one. Individuals categorized by normal and high body fat percentages were used. The DC and CK were proven to be the most advantageous out of the exosome isolation methods, so we suggest these methods for further protein and molecular analyses, respectively. Still, we emphasize the importance of selecting an appropriate methodology depending on the specific research objectives. At the same time, no statistical differences in exosome quality, morphology, total protein, or microRNA concentration were observed between individuals categorized by body fat percentage, so we suggest that the exosomal cargo varies in individuals with normal and high fat percentages.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Plasma exosomes ; Exosome isolation methods ; Fat percentage
Published in:	Life, Vol. 15 Núm. 3 (march 2025) , p. 410, ISSN 2075-1729

DOI: 10.3390/life15030410
PMID: 40141758

11 p, 1.2 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Health sciences and biosciences > Institut de Biotecnologia i de Biomedicina (IBB)
Articles > Research articles
Articles > Published articles